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An Optogenetic Approach for Assessing Formation of Neuronal Connections in a Co-culture System

DOI :

10.3791/52408-v

11:22 min

February 17th, 2015

February 17th, 2015

12,973 Views

1Neuroscience & Behavioral Disorders, Duke-NUS Graduate Medical School, 2Lee Kong Chian School of Medicine, Nanyang Technological University

A protocol to generate a co-culture system consisting of neurons derived from induced pluripotent stem cells (iPSCs), primary cortical neurons and astrocytes is described. This co-culture system allows detection of the formation of synaptic contacts and circuits between new, iPSC-derived neurons and pre-existing cortical neurons expressing channelrhodopsin-2.

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Optogenetic Approach

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