Differentiating Induced Neural Progenitor Cells into Astrocytes

Take a culture of induced neural progenitor cells, or iNPCs, on an extracellular matrix protein-coated multi-well plate.

Replace the media with glial induction media and incubate. Growth factors and hormones in the media facilitate iNPC differentiation into glial precursor cells.

Change the media every other day for cell proliferation. Then, replace the media with astrocyte media and incubate.

Specific nutrients and growth factors in the media drive glial precursor cell differentiation into astrocytes.

Media changes every other day promote astrocyte maturation.