Generating Cortical Pyramidal Neurons from Human Neural Stem Cells

To prepare neuronal cultures, place glass coverslips into six-well culture dishes, and add polyornithine. Incubate overnight under a flow hood. The following day, use DPBS to wash the coverslips three times and add laminin. Incubate under a flow hood for at least 10 hours.

Prepare culture medium containing the following components. Then, use the medium to plate and dispatch neural stem cells or NSCs at a density of 50,000 cells per square centimeter, pipetting with slow rotating movements in order to reduce cell clustering.