JoVE Logo
Faculty Resource Center

Sign In

Preparation of Neuronal Cultures from Midgastrula Stage Drosophila Embryos

DOI :

10.3791/226-v

July 4th, 2007

July 4th, 2007

8,639 Views

1Department of Development and Cell Biology, Department of Anatomy and Neurobiology, University of California, Irvine (UCI)

This video demonstrates the preparation of primary neuronal cultures from midgastrula stage Drosophila embryos. Views of live cultures show cells 1 hour after plating and differentiated neurons after 2 days of growth in a bicarbonate-based defined medium. The neurons are electrically excitable and form synaptic connections.

Tags

Neuronal Cultures

-- Views

Related Videos

article

Primary Neuronal Cultures from the Brains of Late Stage Drosophila Pupae

article

Placing Growth Factor-Coated Beads on Early Stage Chicken Embryos

article

In Ovo Electroporations of HH Stage 10 Chicken Embryos

article

Neuronal Cell Cultures from Aplysia for High-Resolution Imaging of Growth Cones

article

The Preparation of Drosophila Embryos for Live-Imaging Using the Hanging Drop Protocol

article

Preparation of Aplysia Sensory-motor Neuronal Cell Cultures

article

Preparation of embryos for Electron Microscopy of the Drosophila embryonic heart tube

article

In-vivo Centrifugation of Drosophila Embryos

article

Upright Imaging of Drosophila Embryos

article

Primary Cell Cultures from Drosophila Gastrula Embryos

JoVE Logo

Privacy

Terms of Use

Policies

Research

Education

ABOUT JoVE

Copyright © 2024 MyJoVE Corporation. All rights reserved