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Primary Neuronal Cultures from the Brains of Late Stage Drosophila Pupae

DOI :

10.3791/200-v

May 28th, 2007

May 28th, 2007

14,801 Views

1Department of Development and Cell Biology, Department of Anatomy and Neurobiology, University of California, Irvine (UCI)

This video demonstrates the preparation of primary neuronal cultures from the brains of late stage Drosophila pupae. Views of live cultures show neurite outgrowth and imaging of calcium levels using Fura-2.

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Primary Neuronal Cultures

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