JoVE Logo

Sign In

A subscription to JoVE is required to view this content.

In Vivo Electrophysiological Recordings of Brain Activities from Multiple Sites in Rats

-- views • 1:13 min

Transcript

Begin with an anesthetized rat secured in a stereotactic frame with its skull exposed.

Use reference points on the skull to calibrate and mark the desired coordinates.

Drill holes at the marked locations.

Remove debris and cut open the brain's outer layer.

Frequently flush the holes with saline to prevent drying.

Drill additional holes and anchor screws to support the electrodes.

Install the reference electrodes and electrophysiological recording electrodes. Secure them by applying acrylic resin.

Arrange tungsten microwire electrodes on a frame according to the multi-target locations in the brain.

Multiple electrodes allow simultaneous recording of brain activities from multiple target sites.

Using the reference point on the skull, position the tungsten microwire electrodes.

Gently insert them into the brain.

Configure the recording software to detect brain activity.

Lower the electrodes to the target depth and begin data recording.

article

03:30

In Vivo Electrophysiological Recordings of Brain Activities from Multiple Sites in Rats

Related Videos

44 Views

article

10:24

Electrophysiological and Morphological Characterization of Neuronal Microcircuits in Acute Brain Slices Using Paired Patch-Clamp Recordings

Related Videos

17.2K Views

article

10:31

A Visual Guide to Sorting Electrophysiological Recordings Using 'SpikeSorter'

Related Videos

11.0K Views

article

07:17

Electrophysiological Recordings of Single-cell Ion Currents Under Well-defined Shear Stress

Related Videos

6.1K Views

JoVE Logo

Privacy

Terms of Use

Policies

Research

Education

ABOUT JoVE

Copyright © 2025 MyJoVE Corporation. All rights reserved