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Establishing Embryonic Mouse Neural Stem Cell Culture Using the Neurosphere Assay

DOI :

10.3791/2457-v

January 11th, 2011

January 11th, 2011

24,234 Views

1Department of Anatomical Sciences, Shiraz University of Medical Sciences, Shiraz, Iran , 2Department of Neurosurgery, The University of Florida

This video protocol demonstrates the application of the neurosphere assay for the isolation and expansion of neural stem cells from the ganglionic eminences of embryonic day 14-mouse brain.

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