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Isolation and Expansion of the Adult Mouse Neural Stem Cells Using the Neurosphere Assay

DOI :

10.3791/2393-v

November 20th, 2010

November 20th, 2010

26,447 Views

1 Department of Anatomical Sciences, Shiraz University of Medical Sciences, Shiraz, Iran , 2Department of Neurosurgery, University of Florida

This video protocol demonstrates the neurosphere assay method to generate and expand neural stem cells from the adult mouse periventricular region, and provides technical insights to ensure one can achieve reproducible neurosphere cultures.

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Isolation

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