Transformation of Plasmid DNA into E. coli Using the Heat Shock Method

65.6K Views

07:46 min

August 1st, 2007

DOI :

10.3791/253-v

August 1st, 2007


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Transformation

Chapters in this video

0:04

Introduction

0:38

Preparing for Heat Transformation

1:14

Introducing plasmid DNA into bacteria by heat shock

3:05

Plating transformed bacteria on LB agar plate

5:59

Assessing the transformation efficiency

6:42

Conclusion

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