JoVE Logo
Faculty Resource Center

Sign In

Novel Method of Plasmid DNA Delivery to Mouse Bladder Urothelium by Electroporation

DOI :

10.3791/57649-v

May 3rd, 2018

May 3rd, 2018

8,307 Views

1Department of Molecular, Cell, and Developmental Biology, University of California, Santa Cruz

We describe a novel method for the delivery of DNA plasmid into the urothelial cells of mouse bladder in vivo through urethra catheterization and electroporation. It offers a fast and convenient way for generating autochthonous mouse models of bladder diseases.

Tags

Plasmid DNA Delivery

-- Views

Related Videos

article

Purifying Plasmid DNA from Bacterial Colonies Using the Qiagen Miniprep Kit

article

Transformation of Plasmid DNA into E. coli Using the Heat Shock Method

article

DNA Transfection of Mammalian Skeletal Muscles using In Vivo Electroporation

article

Primary Culture and Plasmid Electroporation of the Murine Organ of Corti.

article

Streamlined Purification of Plasmid DNA From Prokaryotic Cultures

article

Direct Delivery of MIF Morpholinos Into the Zebrafish Otocyst by Injection and Electroporation Affects Inner Ear Development

article

A Practical and Novel Method to Extract Genomic DNA from Blood Collection Kits for Plasma Protein Preservation

article

A Novel Ex vivo Culture Method for the Embryonic Mouse Heart

article

In Vivo 4-Dimensional Tracking of Hematopoietic Stem and Progenitor Cells in Adult Mouse Calvarial Bone Marrow

article

Plasmid-derived DNA Strand Displacement Gates for Implementing Chemical Reaction Networks

JoVE Logo

Privacy

Terms of Use

Policies

Research

Education

ABOUT JoVE

Copyright © 2024 MyJoVE Corporation. All rights reserved