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Inducing a Site Specific Replication Blockage in E. coli Using a Fluorescent Repressor Operator System

DOI :

10.3791/54434-v

August 21st, 2016

August 21st, 2016

8,723 Views

1School of Environmental and Life Sciences, University of Newcastle

We describe here a system utilizing a site-specific, reversible in vivo protein block to stall and collapse replication forks in Escherichia coli. The establishment of the replication block is evaluated by fluorescence microscopy and neutral-neutral 2-dimensional agarose gel electrophoresis is used to visualize replication intermediates.

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