Name: a microfluidic device with groove patterns for studying cellular behavior
Uploaded: 2007-08-30T00:00:00
Description: Watch this Scientific Journal Video about A Microfluidic Device with Groove Patterns for Studying Cellular Behavior at JoVE.com

<p class="jove_step"> A.微流体装置的微细加工</p><ol><li> 4英寸硅片的处理与活性氧离子（Harrick科学30W，5分钟，NY）。 </li><li>负型光刻胶（SU - 8 2015年，Microchem，MA）是在一个硅晶片上的1分钟900转的旋涂。 </li><li>晶圆是软烤95℃，在烤盘6分钟，通过一个面罩含微膜4分钟，以紫外灯（200W）。 </li><li>晶圆是出炉后在95℃，6分钟和开发利用SU - 8光刻胶开发。 </li><li>含微的光致抗蚀剂图形晶片被放置在一个Petri盘。 </li><li>聚?...

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	<li>Chung, B. G., Park, J. W., Hu, J. S., Huang, C., Monuki, E. S., Jeon, N. L. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=A+hybrid+microfluidic-vacuum+device+for+interfacing+with+conventional+cell+culture+platform.">A hybrid microfluidic-vacuum device for interfacing with conventional cell culture platform.</a> <em style='font-style: italic'>BMC Biotechnol</em>. <b>7</b>, (2007).</li><li>Khademhosseini, A., Yeh, J., Eng, G., Karp, J., Kaji, H., Borenstein, J., Farokhzad, O. C., Langer, R. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Cell+docking+inside+microwells+within+reversibly+sealed+microfluidic+channels+for+fabricating+multiphenotype+cell+arrays.">Cell docking inside microwells within reversibly sealed microfluidic channels for fabricating multiphenotype cell arrays.</a> <em style='font-style: italic'>Lab Chip</em>. <b>5</b>, 1380-1386 (2005).</li><li>Whittemore, E. R., Loo, D. T., Watt, J. A., Cotman, C. W. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=A+detailed+analysis+of+hydrogen+peroxide-induced+cell+death+in+primary+neuronal+culture.">A detailed analysis of hydrogen peroxide-induced cell death in primary neuronal culture.</a> <em style='font-style: italic'>Neuroscience</em>. <b>67</b>, 921-932 (1995).</li></ol>

<p>细胞固定，并在microgrooved基板图案中的微流体装置。暴露在过氧化氢的细胞凋亡过程中实时观察和分析，通过Annexin V和碘化丙啶。因此，这种微流体装置，包含细纹渠道，可用于高通量药物筛选。 </p>

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		<th>Catalogue Number</th>
		<th>Comment</th>
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<td>PDMS</td>
<td>Reagent</td>
<td>K.R. Anderson Co. Inc. </td>
<td>2065622 </td>
<td>Poly(dimethylsiloxane), Dow Corning Sylgard 184 (8.6 lb)</td>
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<td>DMEM</td>
<td>medium</td>
<td>Invitrogen</td>
<td>11965 </td>
<td>Dulbecco&#x2019;s Modified Eagle&#x2019;s Media </td>
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<td>FBS</td>
<td>serum</td>
<td>Invitrogen</td>
<td>10082-147 </td>
<td>Fetal Bovine Serum
</td>
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<td>Hydrogen peroxide</td>
<td>Reagent</td>
<td>Sigma Aldrich</td>
<td> H1009 </td>
<td>&nbsp;</td>
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<td>&nbsp;</td>
<td>Invitrogen</td>
<td>V13242 </td>
<td>Annexin A, propidium iodide</td>
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<td>&nbsp;</td>
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<td>&nbsp;</td>
<td>4 inch silicone wafer</td>
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<td>Reactive oxygen plasma </td>
<td>Reagent</td>
<td>Harrick Scientific</td>
<td>&nbsp;</td>
<td>treat wafer 5 min at 30W</td>
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<td>inverted microscope </td>
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<td>Nikon </td>
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a microfluidic device with groove patterns for studying cellular behavior

<p class="jove_content">我们描述了一个微流体装置与microgrooved模式，研究细胞行为。这种微流体平台包括一个顶级的流体通道和自下而上microgrooved基板。编造microgrooved渠道，一个顶级的聚二甲基硅氧烷（PDMS）模具包含微流体通道的印象是对齐和粘结到microgrooved基板。使用这种设备，小鼠成纤维细胞被固定在microgrooved基板图案（25，50，75，宽100微米）。为了研究细胞凋亡在微流体装置，介质中含有过氧化氢，膜联蛋白V和碘化丙啶灌注到2小时的流体通道。我们发现，暴露在氧化应激的细胞变得凋亡。通过Annexin V结合在细胞凋亡过程中的质膜外单张磷脂酰证实这些细胞凋亡。 microgrooved模式使用这种微流体装置，细胞凋亡过程中实时观察，并包含一个孵化室<sub>（37℃，5％</sub>的CO 2）使用倒置显微镜分析。因此，这microgrooved基板中的微流体装置可用于研究细胞行为和进行高通量药物筛选。 </p>

Watch this Scientific Journal Video about A Microfluidic Device with Groove Patterns for Studying Cellular Behavior at JoVE.com

A Microfluidic Device with Groove Patterns for Studying Cellular Behavior

<p class="jove_content">我们描述了一种用于制造微流体装置，可以使细胞的捕获和文化协议。在这种方法中，如微流体通道内的沟槽图案的微观结构是用来建立低剪切应力区域内的细胞可以停靠。</p>

微流体装置与Groove的模式，为研究细胞行为

We describe a microfluidic device with microgrooved patterns for studying cellular behavior.  This microfluidic platform consists of a top fluidic channel ...

Research

JoVE Journal

Biology

Fabrication of a Microfluidic Device for the Compartmentalization of Neuron Soma and Axons

条块索玛的神经元和轴突的一个微流体设备制造

In this video, we demonstrate the technique of soft lithography with polydimethyl siloxane (PDMS) which we use to fabricate a microfluidic device for ...

科研

生物学

A Gradient-generating Microfluidic Device for Cell Biology

一个渐变产生的微流体装置，细胞生物学

 The fabrication and operation of a gradient-generating microfluidic device for studying cellular behavior is described.  A microfluidic platform is an ...

Preparing E18 Cortical Rat Neurons for Compartmentalization in a Microfluidic Device

准备在微流体装置E18条块皮质大鼠神经元

In this video, we demonstrate the preparation of E18 cortical rat neurons. E18 cortical rat neurons are obtained from E18 fetal rat cortex previously ...

Cell Capture Using a Microfluidic Device

细胞捕获使用一个微流体装置

Assessing Neural Stem Cell Motility Using an Agarose Gel-based Microfluidic Device

使用琼脂糖凝胶为基础的微流体装置的评估神经干细胞的运动

While microfluidic technology is reaching a new level of maturity for macromolecular assays, cell-based assays are still at an infant stage1. This is ...

Microinjection Techniques for Studying Mitosis in the <em>Drosophila melanogaster</em> Syncytial Embryo

Microinjection Techniques for Studying Mitosis in the Drosophila melanogaster Syncytial Embryo

研究有丝分裂的显微注射技术<em>果蝇</em>合胞体胚胎

This protocol describes the use of the Drosophila melanogaster syncytial embryo  for studying mitosis1.  Drosophila has useful genetics with a sequenced ...

A Multi-Parametric Islet Perifusion System within a Microfluidic Perifusion Device

一个多参数的胰岛内的微流控Perifusion装置Perifusion系统

A microfluidic islet perifusion device was developed for the assessment of dynamic insulin secretion of multiple islets and simultaneous fluorescence ...

Window on a Microworld: Simple Microfluidic Systems for Studying Microbial Transport in Porous Media

窗口上的一个微观世界：简单的微流体系统为研究多孔介质中的微生物运输

Microbial growth and transport in porous media have important implications for the quality of groundwater and surface water, the recycling of nutrients in ...

A Microfluidic Device for Quantifying Bacterial Chemotaxis in Stable Concentration Gradients

一个微流体装置量化细菌趋稳定的浓度梯度

Chemotaxis allows bacteria to approach sources of attractant chemicals or to avoid sources of repellent chemicals. Bacteria constantly monitor the ...

Genomic MRI - a Public Resource for Studying Sequence Patterns within Genomic DNA

磁共振成像基因 - 公共资源为研究基因组DNA序列内模式

Non-coding genomic regions in complex eukaryotes, including intergenic areas, introns, and untranslated segments of exons, are profoundly non-random in ...