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DOI :
10.3791/271-v
August 30th, 2007
Chapters
7:13
Application of reagents
3:20
Cutting layers of microfluidic device
6:55
Experiment: effect of EGF gradient on cell migration
3:33
Creating channel inlet and outlet
0:21
Microfabrication of microfluidic device
3:19
Assembly of microfluidic device
1:49
Patterning PDMS molds
2:20
Removing bubbles by vacuum
3:59
Bonding the microfluidic device layers
9:49
Experiment: cell migration
0:05
Introduction
0:56
Preparation of PDMS mixture
我々は明確に定義された微小環境における空間的および時間的勾配を生成することができる勾配を発生するマイクロ流体デバイスの微細加工のためのプロトコルについて説明します。このアプローチでは、勾配を発生するマイクロ流体デバイスは、指示細胞の遊走、胚形成、創傷治癒、癌の転移を研究するために使用することができます。
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