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A System for Culturing Iris Pigment Epithelial Cells to Study Lens Regeneration in Newt

DOI :

10.3791/2713-v

6:56 min

June 22nd, 2011

June 22nd, 2011

11,386 Views

1Department of Biology, University of Dayton, 2Center for Tissue Regeneration and Engineering, University of Dayton

In newt, the lens regenerates always from the dorsal iris by transdifferentiation of the iris pigmented epithelial cells (IPEs). Here we describe a procedure to culture dorsal and ventral newt IPE cells and their implantation to the newt eye. The implanted cells are then studied by tissue sectioning and immunohistochemistry.

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Iris Pigment Epithelial Cells

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