Name: gene transfer for ischemic heart failure in a preclinical model
Uploaded: 2011-05-15T14:00:00
Description: Watch this Scientific Journal Video about Gene Transfer for Ischemic Heart Failure in a Preclinical Model at JoVE.com

<p class="jove_content"> Leducq基金会通过Caerus网络（RJH），支持这项工作是由美国国立卫生研究院R01 HL093183，HL088434，HL071763，HL080498，HL083156和P20HL100396（RJH）。 DL是由德国研究基金会的支持。我们要感谢她在这个项目的技术援助，劳伦Leonardson。 </p>

<p  class="jove_title"> 1。心肌梗死的创作</p ><ol><li>女约克夏猪，动物生物技术产业（多伊尔斯敦，PA）（20KG）使用Telazol（tiletamine / zolazepam）（8.0毫克/公斤）和丁丙诺啡（0.6毫克）的前药。 </li><li>动物插管和100％的氧气，然后通风。整个过程中，全身麻醉维持异丙酚（5-8mg/kg/hr）。 </li><li>股的网站是准备用70％异丙醇，碘伏酒精。一个经皮穿刺提供访问动脉鞘的位置。鞘插入后，3000-5000 IU的肝素是管理四，维持活化?...

<ol>
	<li>Perez de Prado, A. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Closed-chest+experimental+porcine+model+of+acute+myocardial+infarction-reperfusion.">Closed-chest experimental porcine model of acute myocardial infarction-reperfusion.</a> <em style='font-style: italic'>J Pharmacol Toxicol Methods</em>. <b>60</b>, 301-306 (2009).</li><li>Angeli, F. S. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Left+ventricular+remodeling+after+myocardial+infarction:+characterization+of+a+swine+model+on+beta-blocker+therapy.">Left ventricular remodeling after myocardial infarction: characterization of a swine model on beta-blocker therapy.</a> <em style='font-style: italic'>Comp Med</em>. <b>59</b>, 272-279 (2009).</li><li>Urdaneta, F., Lobato, E. B., Kirby, D. S. &. a. m. p. ;. a. m. p., Sidi, A. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Treating+myocardial+stunning+randomly,+with+either+propofol+or+isoflurane+following+transient+coronary+occlusion+and+reperfusion+in+pigs.">Treating myocardial stunning randomly, with either propofol or isoflurane following transient coronary occlusion and reperfusion in pigs.</a> <em style='font-style: italic'>Ann Card Anaesth</em>. <b>12</b>, 113-121 (2009).</li><li>Jaski, B. E. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Calcium+upregulation+by+percutaneous+administration+of+gene+therapy+in+cardiac+disease+(CUPID+Trial),+a+first-in-human+phase+1/2+clinical+trial.">Calcium upregulation by percutaneous administration of gene therapy in cardiac disease (CUPID Trial), a first-in-human phase 1/2 clinical trial.</a> <em style='font-style: italic'>J Card Fail</em>. <b>15</b>, 171-181 (2009).</li></ol>

<p class="jove_content">在各种原因引起的心力衰竭，缺血是由远在最常见的病因。为了克服这一严重的疾病，各种治疗方法正在制定中。需要仔细评估的疗法之前，这些有前途的疗法是可以应用到诊所。因此，建立的缺血性心力衰竭模型表示患者的临床情况，心力衰竭治疗的发展是至关重要的。我们的猪心肌梗死模型满足这些翻译的要求。 </p><p class="jove_content">要通过创建一个心肌梗死诱发心力衰竭，心肌梗死面积的?...

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		<thead>
		<tr>
		<th>Material Name</th>
		<th>Type</th>
		<th>Company</th>
		<th>Catalogue Number</th>
		<th>Comment</th>
		</tr>
		</thead>
		<tbody>
		
<tr>
<td>Telazol</td>
<td>&nbsp;</td>
<td>Fort Dodge Animal Health</td>
<td>0856-9050-49</td>
<td>&nbsp;</td>
<tr>
<td>Propofol</td>
<td>&nbsp;</td>
<td>APP Pharmaceuticals</td>
<td>63323-270-20</td>
<td>&nbsp;</td>
<tr>
<td>Heparin</td>
<td>&nbsp;</td>
<td>APP Pharmaceuticals</td>
<td>63323-540-11</td>
<td>&nbsp;</td>
<tr>
<td>Amiodarone</td>
<td>&nbsp;</td>
<td>APP Pharmaceuticals</td>
<td>63323-616-03</td>
<td>&nbsp;</td>
<tr>
<td>Atropin Sulfate</td>
<td>&nbsp;</td>
<td>Med-Pharmex</td>
<td>54925-063-10</td>
<td>&nbsp;</td>
<tr>
<td>Potassium acetate</td>
<td>&nbsp;</td>
<td>American Regent, Inc.</td>
<td>2053-25</td>
<td>&nbsp;</td>
<tr>
<td>Nitroglycerine</td>
<td>&nbsp;</td>
<td>American Regent, Inc.</td>
<td>4810-25 </td>
<td>&nbsp;</td>
<tr>
<td>Salix (Furosemide)</td>
<td>&nbsp;</td>
<td>Intervet Inc.</td>
<td>710461</td>
<td>&nbsp;</td>
<tr>
<td>5 Fr Hockey stick catheter</td>
<td>&nbsp;</td>
<td>Cordis</td>
<td>556-278-0L</td>
<td>&nbsp;</td>
<tr>
<td>7 Fr Hockey stick catheter</td>
<td>&nbsp;</td>
<td>Cordis</td>
<td>778-278-00</td>
<td>&nbsp;</td>
<tr>
<td>Coronary wire</td>
<td>&nbsp;</td>
<td>Abbott Laboratories</td>
<td>1044588</td>
<td>&nbsp;</td>
<tr>
<td>Coronary balloon</td>
<td>&nbsp;</td>
<td>Abbott Laboratories</td>
<td>1009447-08</td>
<td>&nbsp;</td>
<tr>
<td>8Fr sheath</td>
<td>&nbsp;</td>
<td>TERUMO</td>
<td>RSS801</td>
<td>&nbsp;</td>		</tbody>
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gene transfer for ischemic heart failure in a preclinical model

<p class="jove_content">各种新兴的技术正在发展为心衰患者。建立良好的临床前评估是必要的，以确定其疗效和安全性。 </p><p class="jove_content">使用病毒载体的基因治疗是治疗心脏疾病的最有前途的的的方法之一。提供各种不同的基因的病毒载体基因通过改变具有不可估量的治疗潜力。 </p><p class="jove_content">在这段视频中，基因转移创造心力衰竭的动物模型的全过程，提出了利用猪模型。第一，阻断近端的左冠状动脉前降支建立心肌梗死。慢性心功能不全心肌重塑的结果。我们的模型的独特之处是一个相当大的疤痕，真正体现严重心力衰竭的病人谁需要积极治疗取得积极成果。心肌梗死后瘢痕组织的创建和发展，同时硝化甘油输液内注射病毒表现。我们的注射方法提供了简单而有效的基因转移，基因表达增强。心肌梗死冠脉内传播病毒的猪模型的结合已被证明是一个一致的和可重复性的方法，这不仅有助于个人基因测试效果，但也比较的许多基因治疗候选人的疗效。 </p>

Watch this Scientific Journal Video about Gene Transfer for Ischemic Heart Failure in a Preclinical Model at JoVE.com

Gene Transfer for Ischemic Heart Failure in a Preclinical Model

<p class="jove_content">一个基因转染对缺血性心力衰竭的治疗方法是用一个猪心肌梗死模型。我们的简单和可重复性的方法，使我们能够很容易用一个非常简单和重复性的方式评估各种基因转移的疗效。</p>

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The development of acute right heart failure (ARHF) in the context of chronic pulmonary hypertension (PH) is associated with poor short-term outcomes. The ...

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Heat stroke is the most severe manifestation of heat-related illnesses. Classic heat stroke (CHS), also known as passive heat stroke, occurs at rest, ...

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