Name: cannulation of the mouse submandibular salivary gland via the wharton's duct
Uploaded: 2011-05-14T14:00:00
Description: Watch this Scientific Journal Video about Cannulation of the Mouse Submandibular Salivary Gland via the Wharton's Duct at JoVE.com

作者感谢技术咨询安娜Cotrim博士和博士Monzur设备Murshed。这项工作是由加拿大卫生研究院资助部分。

1。程序说明<ol><li>皮下注射每只小鼠注入1μL/ g体重0.5毫克/毫升硫酸阿托品水合物（＃11330猫，Fluka公司，美国），以防止破坏输注的运动唾液腺分泌物。 </li><li>麻醉注射1μL/ g的体重为100毫克/毫升氯胺酮和20毫克/毫升甲苯噻嗪（凤凰科学，AR，美国）解决方案的每鼠腹腔（知识产权） </li><li>在腹侧的位置上放置定做的塑料平台的鼠标被锁定在一个金属丝，上颌切牙和下颌切?...

<ol>
	<li>Fox, P. C. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Acquired+salivary+dysfunction.+Drugs+and+radiation.">Acquired salivary dysfunction. Drugs and radiation.</a> Ann N Y Acad Sci. 842, 132-137 (1998).</li><li>Bhide, S. A., Miah, A. B., Harrington, K. J., Newbold, K. L., Nutting, C. M. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Radiation-induced+xerostomia:+pathophysiology,+prevention+and+treatment.">Radiation-induced xerostomia: pathophysiology, prevention and treatment.</a> Clin Oncol (R Coll Radiol). 21, 737-744 (2009).</li><li>Redman, R. S., Ball, W. D., Mezey, E., Key, S. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Dispersed+donor+salivary+gland+cells+are+widely+distributed+in+the+recipient+gland+when+infused+up+the+ductal+tree.">Dispersed donor salivary gland cells are widely distributed in the recipient gland when infused up the ductal tree.</a> Biotech Histochem. 84, 253-260 (2009).</li></ol>

使用此插管方法是一个困难的注入解决方案，以防止回流。台盼蓝溶液，即使暂时成功地迁移到唾液腺，唾液分泌可能会干扰与输液，造成回流的台盼蓝溶液腺体。作为一项预防措施，除使用阿托品抑制唾液的分泌，聚乙烯套管应保持在沃顿商学院的管道和60秒后举行的注入已存入的注射器活塞。这将使大部分的注入的解决方案保持腺体内。 按照惯例，全身应用，即静脉注射...

<table border="1"><tbody><tr><td> 试剂名称 </td><td> 类型 </td><td> 公司 </td><td> 目录编号 </td><td> 评论（可选） </td></tr><tr><td>阿托品</td><td>药物</td><td> Fluka公司</td><td> 11330 </td><td>为了抑制唾液分泌</td></tr><tr><td>氯胺酮</td><td>药物</td><td>凤凰科学</td><td></td><td>镇静</td></tr><tr><td>甲苯噻嗪</td><td>药物</td><td>凤凰科学</td><td></td><td></td></tr><tr><td>聚乙烯管材（直径0.58毫米） </td><td>管</td><td> Intramedic PE - 10，碧迪诊断系统</td><td> 427410 </td><td>要插入比针尖插入到沃顿商学院的管道，以避免在组织损伤。 </td></tr><tr><td>超舒适胰岛素注射器（带29G针） </td><td>注射器</td><td>泰科医疗</td><td> 600145 </td><td></td></tr><tr><td> 4％台盼蓝</td><td>污点</td><td> Gibco公司</td><td> 15250 </td><td></td></tr></tbody></table>

cannulation of the mouse submandibular salivary gland via the wharton's duct

在干燥综合征，并接受他们的头部和颈部区域，用于治疗癌症的治疗照射患者经常发现严重唾液腺功能减退症。两组患者出现症状，如口干（口干），吞咽困难（咀嚼和吞咽障），严重龋齿，味觉改变，口咽感染（念珠菌），口腔粘膜炎，疼痛和不适。 在RS雷德曼，2009年发送Mezey等再生医学的一个创新的方法治疗的唾液腺低功能推测：中，干细胞可以直接存入腺体由插管在恢复受损的功能的有力方法器官。据推测，移居国外的干细胞会分化成腺细胞作为主机唾液腺的一部分功能。此外，这种插管技术是一种快捷和有效地用于临床的基因转移中的应用的传递方法。 在这里，我们说明了沃顿商学院的管（ 图1）通过对小鼠颌下腺唾液腺在执行插管过程中所涉及的步骤。 C3H鼠（查尔斯河，蒙特利尔，QC，加拿大）用于本实验中，已清洁常规条件下保持在麦吉尔大学动物资源中心。所有的实验已经大学动物保健委员会批准，并按照加拿大动物保健委员会的指引。 在这个实验中，台盼蓝溶液注入腺体内，通过使用胰岛素注射器与一个29号针头包裹聚乙烯管内一个开放的沃顿商学院的管。随后，鼠标解剖显示，输注成功迁移进入腺体。

Watch this Scientific Journal Video about Cannulation of the Mouse Submandibular Salivary Gland via the Wharton's Duct at JoVE.com

Cannulation of the Mouse Submandibular Salivary Gland via the Wharton's Duct

鼠标颌下唾液腺通过沃顿商学院的导管插管协议描述。在这个实验中，台盼蓝溶液作为一个代尔，证明这种技术如何有效地注入到有针对性的腺，并建议作为一个潜在的临床药物/唾液腺的再生细胞治疗这种新方法的可靠性。

沃顿商学院的导管插管通过小鼠颌下腺唾液腺

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