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DOI :
10.3791/3089-v
•
10:45 min
October 13th, 2011
Chapters
0:05
Title
1:44
Establishment of Fibroblast Cultures from Skin Explants
2:57
Stage I: Preparation of Collagen I from Rat Tails
4:55
Stage II: Setting Up the 3D Matrix with Embedded Fibroblasts
6:48
Stage III: Plating Cells of Interest on Top of the Matrix
7:33
Stage IV: Transferring the Matrix to a Grid for Invasion (approximately 0-21 days)
8:38
Stage V: Fixation
9:02
Applications of the Organotypic Assay
10:18
Conclusion
描述的方法,用于制备一个3 - 维的矩阵组成的I型胶原蛋白和初级人成纤维细胞。器官凝胶作为一个有用的基体,以评估侵入细胞的迁移,因为它模仿组织基质的基本功能,是适合各种形式的显微镜。
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