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DOI :
10.3791/3089-v
•
10:45 min
October 13th, 2011
Chapters
0:05
Title
1:44
Establishment of Fibroblast Cultures from Skin Explants
2:57
Stage I: Preparation of Collagen I from Rat Tails
4:55
Stage II: Setting Up the 3D Matrix with Embedded Fibroblasts
6:48
Stage III: Plating Cells of Interest on Top of the Matrix
7:33
Stage IV: Transferring the Matrix to a Grid for Invasion (approximately 0-21 days)
8:38
Stage V: Fixation
9:02
Applications of the Organotypic Assay
10:18
Conclusion
メソッドは、I型コラーゲンと初代ヒト線維芽細胞からなる3次元マトリックスの調製について記載されています。この器官ゲルは、それが組織の間質の基本的な機能を模倣し、顕微鏡の多くの形態が可能であるため、侵襲的な細胞遊走を評価するために有用な基質として機能します。
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