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DOI :
10.3791/3123-v
July 28th, 2011
Chapters
0:05
Title
1:34
Labeling of Pathogens with Fluorescent Dye
2:52
Preparation of RAW 264.7 Macrophages for Chamber Slides
4:04
Selection and Manipulation of a Pathogen with the Optical Trap
6:18
Representative Images of Pathogen and Immune Cell Interactions After Trapping-Initiated Pathogen:Cell Contact
8:35
Conclusion
メソッドは、個別に、選択した操作、および回転するディスクの顕微鏡に結合される光トラップを使用して画像ライブの病原体に記述されています。光トラップは、生物の空間的、時間的な制御を提供し、宿主細胞にそれらが隣接して配置されます。蛍光顕微鏡は、細胞への最小限の摂動で動的な細胞間相互作用をキャプチャします。
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