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DOI :
10.3791/3200-v
•
9:25 min
September 15th, 2011
Chapters
1:37
PORN-H/Laminin Coating of Dishes and Coverslips
0:05
Title
2:33
Lectin Coating of the Purification Plate
3:20
Motoneuron Culture Medium
4:32
Spinal Cord Dissection
5:35
Enrichment of Motoneurons by Lectin-based Purification
7:28
Results: Lectin-based Isolation and Characterization of Mouse Embryonic Motoneurons
8:46
Conclusion
脊髄から、マウス胚性運動ニューロンを分離する別の方法が説明されています。方法は、考慮にレクチンが低親和性神経成長因子受容体p75NTRにバインドすることができるという事実を取ります。このレクチンベースpreplatingはp75NTRに対する特異的抗体を持つと同様の精製が可能になります。
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