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DOI :
10.3791/3343-v
•
7:19 min
September 28th, 2011
Chapters
0:05
Title
1:20
Experimental Design and GoClone 3'UTR Reported Selection
2:36
Co-transfection of 3' UTR Reporters and microRNA
5:00
Measurement of Luciferase Activity with LightSwitch Assay Reagents
5:48
Luciferase Knockdown Assay: Analysis and Representative Results
6:54
Conclusion
微RNA(miRNA)是基因表达的重要调节因子,已被证明在许多生物过程中发挥的作用。为了更好地了解miRNA的UTR区的相互作用,我们已经创建了一个全基因组的3 UTR的荧光素酶记者的集合,搭配了一种新的荧光素酶基因的检测试剂,LightSwitch系统。
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