Name: morphometric analyses of retinal sections
Uploaded: 2012-02-19T14:00:00
Description: Watch this Scientific Journal Video about Morphometric Analyses of Retinal Sections at JoVE.com

眼科研究在本实验室工作的支持，杜鹃（1972）美国卫生署助理基金会，捐赠基金，香港大学专业进修学院的小项目基金（20097176185）。

1。工具显微镜，尼康立体声调查，医疗保险基金的生物科技公司 - MicroBrightField 2。准备<ol><li>任何形态分析工作之前，每个视网膜样本是在4微米厚的切片，经过H＆E染色。 </li><li>视网膜节分为4个区域 - 上周边地区，中部地区上，较低的周边地区，中部地区较低。 </li><li>后定义的区域，每个区域的图像尼康明亮的光束显微镜10x40倍?...

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	<li>Lam, T. T., Abler, A. S., Tso, M. O. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=N-Methyl-D-Aspartate+(NMDA)â&#x20AC;&#x201C;Induced+Apoptosis+in+Rat+Retina.">N-Methyl-D-Aspartate (NMDA)â&#x20AC;&#x201C;Induced Apoptosis in Rat Retina.</a> Investigative Ophthalmology &amp; Visual Science. 40, 2391-2397 (1999).</li><li>Lam, T. T., Abler, A. S., Tso, M. O. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Apoptosis+and+caspases+after+ischemia-reperfusion+injury+in+rat+retina.">Apoptosis and caspases after ischemia-reperfusion injury in rat retina.</a> Invest. Ophthalmol. Vis. Sci. 40, 967-975 (1999).</li><li>Luo, X. G., Chiu, K., Lau, H. S., Lee, V. W. H., Yung, K. K. L., So, K. F. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=The+Selective+Vulnerability+of+Retinal+Ganglion+Cells+in+Rat+Chronic+Ocular+Hypertension+Model+at+Early+Phase.">The Selective Vulnerability of Retinal Ganglion Cells in Rat Chronic Ocular Hypertension Model at Early Phase.</a> Cellular and Molecular Neurobiology. 29 (8), 1143-1151 (2009).</li><li>Han, Y. S., Chung, I. Y., Park, J. M., Yu, J. M. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Neuroprotective+effect+of+citicoline+on+retinal+cell+damage+induced+by+kainic+acid+in+rats.">Neuroprotective effect of citicoline on retinal cell damage induced by kainic acid in rats.</a> Korean J. Ophthalmol. 19, 219-226 (2005).</li><li>Hernandez, M., Urcola, J. H. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Retinal+ganglion+cell+neuroprotection+in+a+rat+model+of+glaucoma+following+brimonidine,+latanoprost+or+combined+treatments.">Retinal ganglion cell neuroprotection in a rat model of glaucoma following brimonidine, latanoprost or combined treatments.</a> Exp. Eye Res. 86, 798-806 (2008).</li><li>Chan, H. C., Chang, R. C. C., Ip, A. K. C., Chiu, K., Yuen, W. H., Zee, S. Y., So, K. F. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Neuroprotective+effects+of+Lycium+barbarum+Lynn+on+protecting+retinal+ganglion+cells+in+an+ocular+hypertension+model+of+glaucoma.">Neuroprotective effects of Lycium barbarum Lynn on protecting retinal ganglion cells in an ocular hypertension model of glaucoma.</a> Experimental Neurology. 203, 269-273 (2007).</li></ol>

1。如何获得更精确​​的厚度测量？ 您可以放大图像，通过点击“放大”按钮，并留在跟踪窗口点击。 INL的边界可以清楚地看到。如果线的一角，是不是在外部边界的INL的，我们可以调整行的编辑模式下。有关行采摘后，右击跟踪窗口，选择“插入点在选定的轮廓”。有一点可以添加外部边界，而加点应该是在同一直线上。然后，你必须删除原有的点和线可以得到一个更精?...

A correction was made to <a href="http://www.jove.com/video/3377">Morphometric Analyses of Retinal Sections</a>. There was an error with an author's name.

Erratum: Morphometric Analyses of Retinal Sections

<table border="1"><tbody><tr><td> 试剂名称 </td><td> 类型 </td><td> 公司 </td><td> 目录编号 </td></tr><tr><td>立体声调查</td><td>医疗保险基金生命科学分析软件</td><td> MicroBright场</td><td></td></tr><tr><td>显微镜</td><td>奥林巴斯BX51 </td><td>奥林巴斯公司</td><td> BX51 </td></tr></tbody></table>

morphometric analyses of retinal sections

已被用于检查视网膜疾病视网膜节的形态分析。举例来说，神经细胞均显着失去了在大鼠模型中视网膜神经节细胞层（RGCL）与N-甲基-D-天冬氨酸（NMDA）受体诱导的兴奋1，视网膜缺血再灌注损伤的2和3青光眼。 INL和内网状层（IPL），厚度减少了与胞二磷胆碱治疗老鼠的眼睛红藻酸介导的谷氨酸4逆转。修改RGC的密度和体躯大小不同的药物治疗，升高眼内压3,5,6眼睛观察。因此，有客观分析视网膜morphometries的方法可能是在评价视网膜病变和治疗策略的有效性具有十分重要的意义。 视网膜结构是多层次和几种不同类型的神经元EXIST在视网膜上。视网膜细胞数量，细胞的大小和不同层的厚度，如形态参数是比细胞培养系统更复杂。在早期，这些参数可以使用其他的商业成像软件检测。该值通常是相对值，和不断变化的精确值，可能需要进一步的精确计算。此外，细胞大小和形态的跟踪可能不准确，灵敏的统计分析不够，特别是在慢性青光眼模型。在本协议所使用的测量提供了一个更精确和更容易的方式。与细胞的大小和行的绝对长度可以直接和容易被复制到其他文件。例如，我们追踪内层和最外层的INL在核边缘，形成一条线，然后使用该软件绘制成90度角来衡量的厚度。虽然没有软件的帮助，也许斜行和视网膜厚度的变化可能个别观察员之间不重复的。此外，视网膜神经节细胞的数量和密度也可以量化。该协议成功的减少在定量的视网膜功能的变异，增加检测微小变化的敏感性。 这部影片将展示三种类型的视网膜节的形态分析。它们包括测量的INL厚度，定量视网膜神经节细胞的数量和绝对值测量视网膜神经节细胞的大小。这三种分析进行与立体声调查（医疗保险基金生命科学 - 公司MicroBrightField）。该技术可以提供一个简单，但科学的形态分析的平台。

Watch this Scientific Journal Video about Morphometric Analyses of Retinal Sections at JoVE.com

Morphometric Analyses of Retinal Sections

该视频演示了三种类型，其中包括视网膜内的核层厚度测量，定量视网膜神经节细胞（RGCs）的数量，并测量视网膜神经节细胞的大小，形态分析。该技术可以提供一个简单，但科学的形态分析的平台。

视网膜节的形态分析

Morphometric analyses of retinal sections have been used in examining retinal diseases. For examples, neuronal cells were significantly lost in the ...

Research

JoVE Journal

Neuroscience

An Isolated Retinal Preparation to Record Light Response from Genetically Labeled Retinal Ganglion Cells

一个孤立的视网膜制备，记录从基因标记的视网膜神经节细胞的光反应

The first steps in vertebrate vision take place when light stimulates the rod and cone photoreceptors of the retina 1.  This information is then ...

科研

神经科学

Dissection, Culture, and Analysis of Xenopus laevis Embryonic Retinal Tissue

Dissection, Culture, and Analysis of Xenopus laevis Embryonic Retinal Tissue

解剖，文化与分析<em&gt;非洲爪蟾</em&gt;胚胎视网膜组织

The process by which  the anterior region of the neural plate gives rise to the vertebrate retina  continues to be a major focus of both clinical and ...

Automated High-throughput Behavioral Analyses in Zebrafish Larvae

在斑马鱼幼虫的高通量自动化的行为分析

We have created a novel high-throughput imaging system for the analysis of behavior in 7-day-old zebrafish larvae in multi-lane plates. This system ...

Protocol for Three-dimensional Confocal Morphometric Analysis of Astrocytes

协议星形胶质细胞的三维共聚焦形态分析

As glial cells in the brain, astrocytes have diverse functional roles in the central nervous system. In the presence of harmful stimuli, astrocytes modify ...

Immunostaining of Biocytin-filled and Processed Sections for Neurochemical Markers

对于神经化学标记用biocytin填充和加工部分的免疫染色

Electrophysiological recordings of cells using the patch clamp technique have allowed for the identification of different neuronal types based on firing ...

Microstate and Omega Complexity Analyses of the Resting-state Electroencephalography

静止状态脑电图的微状态和欧米茄复杂性分析

Microstate and omega complexity are two reference-free electroencephalography (EEG) measures that can represent the temporal and spatial complexities of ...

An Unbiased Approach of Sampling TEM Sections in Neuroscience

神经科学中透射电镜切片采样的一种无偏方法

Investigations of the ultrastructural features of neurons and their synapses are only possible with electron microscopy. Especially for comparative ...

Preparation of Mouse Retinal Cryo-sections for Immunohistochemistry

小鼠视网膜冷冻节的制备,用于免疫性植物化学

Preparation of high-quality mouse eye sections for immunohistochemistry (IHC) is critical for assessing the retinal structure and function and for ...

Preparation of Newborn Rat Brain Tissue for Ultrastructural Morphometric Analysis of Synaptic Vesicle Distribution at Nerve Terminals

新生大鼠脑组织在神经端子突形圆锥花序分布超微结构形态分析中的制备

Our laboratory and many others have exploited the high resolving power of transmission electron microscopy to study the morphology and spatial ...

Collection of Frozen Rodent Brain Regions for Downstream Analyses

用于下游分析的冷冻啮齿动物大脑区域集合

As our understanding of neurobiology has progressed, molecular analyses are often performed on small brain areas such as the medial prefrontal cortex ...