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DOI :
10.3791/3422-v
•
10:45 min
January 13th, 2012
Chapters
0:05
Title
1:22
Culture and Transfection of Oligodendroglial Cells
4:13
Preparing Cells for Live-cell Image (LCI)
6:21
Treatment of Cells with Apoptosis Inducers and NucView 488 Caspase-3 Substrate
7:20
Live-cell Imaging
8:27
Cleaved Caspase-3 Actitity after Poassium-induced Membrane Depolarization
10:10
Conclusion
活细胞成像的caspase - 3介导的细胞凋亡,永生N19少突胶质细胞培养使用 NucView 488的caspase - 3基板。这种技术适用于多种类型的细胞和组织细胞程序化死亡实时检测。
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