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DOI :
10.3791/3422-v
•
10:45 min
January 13th, 2012
Chapters
0:05
Title
1:22
Culture and Transfection of Oligodendroglial Cells
4:13
Preparing Cells for Live-cell Image (LCI)
6:21
Treatment of Cells with Apoptosis Inducers and NucView 488 Caspase-3 Substrate
7:20
Live-cell Imaging
8:27
Cleaved Caspase-3 Actitity after Poassium-induced Membrane Depolarization
10:10
Conclusion
使用して不死化N19 -オリゴデンドロサイトの培養細胞におけるカスパーゼ-3を介するアポトーシスの生細胞イメージング NucView 488カスパーゼ3基質。この手法は、細胞の種類や組織の様々なリアルタイムでのプログラム細胞死のアッセイに適用可能です。
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