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Reproducible Mouse Sciatic Nerve Crush and Subsequent Assessment of Regeneration by Whole Mount Muscle Analysis

DOI :

10.3791/3606-v

February 22nd, 2012

February 22nd, 2012

32,770 Views

1Center for Neural Repair and Rehabilitation, Temple University

In this report we describe a method to crush mouse sciatic nerve. This method uses readily available hemostatic forceps and easily and reproducibly produces complete sciatic nerve crush. In addition, we describe a method to prepare muscle whole mounts suitable for analysis of nerve regeneration after sciatic nerve crush.

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Reproducible

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