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Whole Mount Dissection and Immunofluorescence of the Adult Mouse Cochlea

DOI :

10.3791/53561-v

January 1st, 2016

January 1st, 2016

52,186 Views

1Department of Surgery, Division of Otolaryngology, Southern Illinois University, School of Medicine, 2Department of Pharmacology, Southern Illinois University, School of Medicine

We present a method to isolate the adult organ of Corti as three intact cochlear turns (apex, middle, and base). We also demonstrate a procedure for immunostaining with fluorescently tagged antibodies. Together these techniques allow the study of hair cells, supporting cells, and other cell types found in the cochlea.

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Whole Mount Dissection

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