JoVE Logo
Faculty Resource Center

Sign In

Alginate Microcapsule as a 3D Platform for Propagation and Differentiation of Human Embryonic Stem Cells (hESC) to Different Lineages

DOI :

10.3791/3608-v

March 9th, 2012

March 9th, 2012

16,030 Views

1Stem Cell Lab, School of Psychiatry, Faculty of Medicine, The University of New South Wales, 2Siriraj Center of Excellence for Stem cell Research, Faculty of Medicine Siriraj Hospital, Mahidol University , 3Neuropsychiatric Institute, Prince of Wales Hospital

We have optimized a microencapsulation technique as an effective 3D platform for propagation and differentiation of embryonic stem cells to endoderm and dopaminergic (DA) neurons. It also provides an opportunity for immune-isolation of cells from the host during transplantation. This platform can be adapted for other cell types.

-- Views

Related Videos

article

Micropatterned Surfaces to Study Hyaluronic Acid Interactions with Cancer Cells

article

Bioluminescence Imaging for Assessment of Immune Responses Following Implantation of Engineered Heart Tissue (EHT)

article

A Simple Hanging Drop Cell Culture Protocol for Generation of 3D Spheroids

article

Alginate Hydrogels for Three-Dimensional Organ Culture of Ovaries and Oviducts

article

The Three-Dimensional Human Skin Reconstruct Model: a Tool to Study Normal Skin and Melanoma Progression

article

Organotypic Collagen I Assay: A Malleable Platform to Assess Cell Behaviour in a 3-Dimensional Context

article

GENPLAT: an Automated Platform for Biomass Enzyme Discovery and Cocktail Optimization

article

Design of a Cyclic Pressure Bioreactor for the Ex Vivo Study of Aortic Heart Valves

article

Parallel-plate Flow Chamber and Continuous Flow Circuit to Evaluate Endothelial Progenitor Cells under Laminar Flow Shear Stress

article

Rotating Cell Culture Systems for Human Cell Culture: Human Trophoblast Cells as a Model

JoVE Logo

Privacy

Terms of Use

Policies

Research

Education

ABOUT JoVE

Copyright © 2024 MyJoVE Corporation. All rights reserved