Name: expanding cytotoxic t lymphocytes from umbilical cord blood that target cytomegalovirus, epstein-barr virus, and adenovirus
Uploaded: 2012-05-07T13:00:00
Description: Watch this Scientific Journal Video about Expanding Cytotoxic T Lymphocytes from Umbilical Cord Blood that Target Cytomegalovirus, Epstein-Barr Virus, and Adenovirus at JoVE.com

Bu çalışma Dan L. Duncan işbirlikçi araştırma bursu (SPK ve EJS), Ulusal Kalp, Akciğer ve Kan Enstitüsü (US4HL081007), bir Lösemi ve Lenfoma Derneği Klinik Araştırmacı Ödülü (SPK) ve Ulusal Kanser Enstitüsü tarafından desteklenen (RO1 CA06150816; EJS).

1. Mononükleer Hücre İzolasyonu (gün 0) <ol><li> Kordon kanı ünitesi% 20 fraksiyonu çıkış portuna dişi luer adaptör başak takın, şırınga takın ve kan çıkarın. 50 ml santrifüj tüpüne ısıtılmış RPMI içinde 20 mL çözülmüş kan aktarın. RPMI 5 mL kordon kan torbası Durulama ve aynı santrifüj tüpüne aktarılır. </li><li> 400 x g hızında 10 dakika için hücreler santrifüjleyin. Süpernatant aspire. </li><li> Sıcak RPMI 20 mL hücreler yeniden süspanse edin. 50 mL santrifüj tü...

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<html> TCMB sonra viral enfeksiyonlar kontrol altına almayı amaçlayan güncel yaklaşımlar etkili olabilir, ancak önemli toksisiteleri ile ilişkili, pahalıdır ve daha sonra enfeksiyona karşı uzun süreli koruma kazandırmaz. Aslında, bazı antiviral ilaçların kullanımı başka koruyucu olacağı virüs-spesifik T hücrelerin genişlemesini sınırlayan olabilir. 14. Başka bir seçenek donör kaynaklı virüs özel T-hücrelerinin bir infüzyon. Biz ve diğerleri gibi T hücreleri İşte, güvenl...

<table border="1"><tbody><tr><td> Reaktif Adı </td><td> Şirket </td><td> Katalog numarası </td></tr><tr><td> RPMI 1640 </td><td> Invitrogen </td><td> 21870-076 </td></tr><tr><td> DC medya </td><td> CellGenix </td><td> 20801-0500 </td></tr><tr><td> EHAA (tıklayın kullanıcısının Orta) </td><td> Irvine Scientific </td><td> 9195 </td></tr><tr><td> İnsan Serum </td><td> İkizler Bio Ürünler </td><td> 100-110 </td></tr><tr><td> Gaz geçirgen Cultureware 18 </td><td> Wilson-Wolf </td><td> 80040S </td></tr><tr><td> IL-2 </td><td> Chiron (TCH Eczane) </td><td></td></tr><tr><td> IL-12 </td><td> NCI / CTEP </td><td></td></tr><tr><td> IL-15 </td><td> CellGenix </td><td> 1013-050 </td></tr><tr><td> IL-7, </td><td> Ar-Ge </td><td> AFL207 </td></tr><tr><td> IL-1beta </td><td> Ar-Ge </td><td> AFL201 </td></tr><tr><td> IL-6, </td><td> HücreGenix </td><td> 1004-050 </td></tr><tr><td> GM-CSF </td><td> TCH Eczane </td><td></td></tr><tr><td> IL-4, </td><td> Ar-Ge </td><td> <a href="http://www.rndsystems.com/pdf/AFL204.pdf" target="_blank">AFL204</a> </td></tr><tr><td> TNF-alfa </td><td> Ar-Ge </td><td> AFL210 </td></tr><tr><td> Ad5f35pp65 </td><td> BCM CAGT Vektör Üretim Tesisi </td><td></td></tr><tr><td> Plazma transferi dişi luer adaptör seti </td><td> Charter Tıp </td><td> 89-550-66j </td></tr><tr><td> Lymphoprep </td><td> Nycomed </td><td> 1114550 </td></tr></tbody></table>

expanding cytotoxic t lymphocytes from umbilical cord blood that target cytomegalovirus, epstein-barr virus, and adenovirus

Kök hücre nakli sonrası Virüs enfeksiyonları, özellikle kordon kanı sonra, ölümün en sık nedenleri arasındadır (CB) transplantasyonu (TCMB) burada CB enfeksiyondan alıcı koruyabilirsiniz virüs deneyimli T hücreleri 1 sezilebilir numaraları içermez. - Biz ve diğerleri, virüs spesifik CTL seropozitif vericilerden elde edilen ve alıcı için infüze olduğunu göstermiştir 4 güvenli ve koruyucu. 5-8 Bununla birlikte, yakın zamana kadar, virüs spesifik T hücreleri dolayı muhtemelen, kordon kan elde edilememiştir virüs özel bellek T hücrelerinin yokluğunda. Daha naif T hücrelerinin in vivo emişli koşullarda taklit çabasıyla, biz böylece T hücre spesifitesi sürüş, CB-türevli dendritik hücreler (DC) immünodominant CMV antijeni pp65 içeren bir adenoviral vektör (Ad5f35pp65) ile transdük kullanılan bir yöntem kurdu inisiyasyon az CMV ve adenovirüs. 9 doğru, biz bu ma kullanınyakalandığı DC&#39;ler gibi her ikisi de ifade IL-7, IL-12 ve IL-15. ikinci biz EBV-dönüştürülmüş B hücreleri uyarımı kullanılmış, ya da EBV-LCL, az 10 sitokinler varlığında CB-alınan T-hücrelerinde latent ve litik EBV antijenleri. Ad5f35pp65-transdük EBV-LCL ikinci stimülasyon da IL-15 varlığında T hücrelerinin uyarmak için kullanılır. Müteakip uyarımlar EBV-LCL ve IL-2 Ad5f35pp65-transduced kullanın. 50x10 6 CB mononükleer hücreler itibaren biz antijenik uyarılara yanıt olarak antijen ile doldurulan hedefleri ve serbest sitokinler lyse 150 x 10 6 virüs-spesifik T hücreleri yukarı oluşturmak edebiliyoruz. 11. Bu hücreler kullanılarak GMP uyumlu şekilde imal edilmiştir yalnızca % 20 bir fraksiyone kordon kanı ünitesi fraksiyonu ve klinik kullanım için tercüme edilmiştir.

Watch this Scientific Journal Video about Expanding Cytotoxic T Lymphocytes from Umbilical Cord Blood that Target Cytomegalovirus, Epstein-Barr Virus, and Adenovirus at JoVE.com

Expanding Cytotoxic T Lymphocytes from Umbilical Cord Blood that Target Cytomegalovirus, Epstein-Barr Virus, and Adenovirus

Burada ilk iyi üretim uygulamaları (GMP) uyumlu göbek kordon kanı, ağırlıklı naif T hücrelerinin kaynağı virüs-spesifik sitotoksik T lenfositler (CTL) üretme yöntemi açıklar.

Kordon Kanı adlı Sitotoksik T lenfositler genişletilmesi olduğunu Hedef Sitomegalovirüs, Epstein-Barr Virus ve Adenovirüs

Virus infections after stem cell transplantation are among the most common causes of death, especially after cord blood (CB) transplantation (CBT) where ...

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Immunology and Infection

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Hazırlanması ve kullanımı, HIV-1 Enfekte İlköğretim CD4 + T-Hücreleri Hedef Hücreler olarak Natural Killer Hücre Sitotoksik Tahliller

 Natural killer (NK) cells are a vital component of the innate immune response to virus-infected cells. It is important to understand the ability of NK ...

Methods to Assess Beta Cell Death Mediated by Cytotoxic T Lymphocytes

Sitotoksik T Lenfositler Beta Hücre Ölümü aracılığı ile değerlendirin yöntemleri

Type 1 diabetes (T1D) is a T cell mediated autoimmune disease.  During the pathogenesis, patients become progressively more insulinopenic as 
insulin ...

Introducing Shear Stress in the Study of Bacterial Adhesion

Bakteriyel Yapışma Çalışma Kesme Stres Tanıtımı

During bacterial infections a sequence of interactions occur between the pathogen and its host. Bacterial adhesion to the host cell surface is often the ...

Establishment of Epstein-Barr Virus Growth-transformed Lymphoblastoid Cell Lines

Epstein-Barr Virus Büyüme dönüştürülmüş lenfoblastoid hücre dizilerinde kurulması

Infection of B cells with Epstein-Barr virus (EBV) leads to proliferation and subsequent immortalization, resulting in establishment of lymphoblastoid ...

Clinical Application of Sleeping Beauty and Artificial Antigen Presenting Cells to Genetically Modify T Cells from Peripheral and Umbilical Cord Blood

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Klinik Uygulama<em&gt; Sleeping Beauty</emGenetiği Periferik ve Göbek Kordon Kanı alınan T hücreleri Değiştir Sunan Hücreler&gt; ve Yapay Antijen

The potency of clinical-grade T cells can be improved by combining gene therapy with immunotherapy to engineer  a biologic product with the potential for ...

Isolation of Precursor B-cell Subsets from Umbilical Cord Blood

Kordon Kanı gelen Prekürsör B-hücreli altkümeleri İzolasyonu

Umbilical cord blood is highly enriched for hematopoietic progenitor cells at different lineage commitment stages. We have developed a protocol for ...

Isolation and Th17 Differentiation of Na&iuml;ve CD4 T Lymphocytes

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İzolasyon ve naif CD4 T Lenfositler Th17 Farklılaşma

Th17 cells are a distinct subset of T cells that have been found to produce interleukin 17 (IL-17), and differ in function from the other T cell subsets ...

Development of an IFN-&#947; ELISpot Assay to Assess Varicella-Zoster Virus-specific Cell-mediated Immunity Following Umbilical Cord Blood Transplantation

Development of an IFN-ÃƒÅ½Ã‚Â³ ELISpot Assay to Assess Varicella-Zoster Virus-specific Cell-mediated Immunity Following Umbilical Cord Blood Transplantation

Göbek Kordon Kanı Transplantasyonu takiben, IFN-γ değerlendirin ELISpot Tahlili Varisella-Zoster Virüs spesifik hücre-aracılı bağışıklık geliştirilmesi

Varicella zoster virus (VZV) is a significant cause of morbidity and mortality following umbilical cord blood transplantation (UCBT). For this reason, ...

Generation of Human Alloantigen-specific T Cells from Peripheral Blood

Periferik Kan İnsan alloantijen-özgü T hücrelerinin üretilmesi

The study of human T lymphocyte biology often involves examination of responses to activating ligands. T cells recognize and respond to processed peptide ...

Radial Mobility and Cytotoxic Function of Retroviral Replicating Vector Transduced, Non-adherent Alloresponsive T Lymphocytes

Radyal Hareket ve retroviral replike olan bir vektör transduse, Yapışmayan Alloresponsive T lenfositlerinin sitotoksik işlev

We report a novel adaptation of the Radial Monolayer Cell Migration assay, first reported to measure the radial migration of adherent tumor cells on ...