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Identification and Characterization of Protein Glycosylation using Specific Endo- and Exoglycosidases

DOI :

10.3791/3749-v

9:54 min

December 26th, 2011

December 26th, 2011

35,942 Views

1New England Biolabs

Using specific glycosidases to remove sugars from glycoproteins followed by SDS-PAGE is a valuable method to detect glycan modifications on protein samples and is a good choice for initial glycobiology studies. Changes following deglycosylation can be detected as shifts in gel mobility or by staining with glycan sensitive reagents.

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Protein Glycosylation

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