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DOI :
10.3791/3803-v
•
12:08 min
March 18th, 2012
Chapters
0:05
Title
1:37
Anaerobic Buffer
2:24
Removing Oxygen from the Stopped-flow System
3:22
Key Solutions
4:45
Reductive Half-reaction: Monitoring Flavin Reduction
7:31
Oxidative Half-reaction: Monitoring Flavin Oxidation
10:10
Stopped Flow: Spectral Forms of Af SidA
11:27
Conclusion
我们描述了一停流仪器的使用调查还原和氧化半反应曲霉一个铁载体(SIDA),黄素依赖型单加氧酶。然后,我们显示相应的在思达反应的物种的光谱,并计算其形成的速率常数。
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