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DOI :
10.3791/3803-v
•
12:08 min
March 18th, 2012
Chapters
0:05
Title
1:37
Anaerobic Buffer
2:24
Removing Oxygen from the Stopped-flow System
3:22
Key Solutions
4:45
Reductive Half-reaction: Monitoring Flavin Reduction
7:31
Oxidative Half-reaction: Monitoring Flavin Oxidation
10:10
Stopped Flow: Spectral Forms of Af SidA
11:27
Conclusion
我々は、還元および酸化半反応の両方を調査するストップトフロー装置の使用を記述アスペルギルスシデロフォアA(SIDA)、フラビン依存性モノオキシゲナーゼ。次にキンゴジカの反応の種に対応するスペクトルを示し、我々はそれらの形成のための速度定数を計算します。
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