Name: wholemount immunohistochemistry for revealing complex brain topography
Uploaded: 2012-04-05T13:00:00
Description: Watch this Scientific Journal Video about Wholemount Immunohistochemistry for Revealing Complex Brain Topography at JoVE.com

RVS understøttes af nye investigator startfonde fra Albert Einstein College of Medicine i Yeshiva University.

1. Dyr Perfusion og cerebellum Dissection <ol><li> Afhængigt af proteinet, kan perfusion være afgørende for vellykket farvning 1,2. Transcardial perfusion er en invasiv, non-overlevelse procedure, der kræver den korrekte brug af anæstetika. Korrekt uddannelse, institutionel godkendelse, og IACUC godkendelse er alle nødvendige, før du forsøger proceduren. Det er altid en god idé at konsultere institutionens dyrlæger for at få hjælp til at identificere eksperimentelle krav og erhverve den rigtige ...

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	<li>Sillitoe, R. V., Hawkes, R. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Whole-mount+Immunohistochemistry:+A+high-throughput+screen+for+patterning+defects+in+the+mouse+cerebellum.">Whole-mount Immunohistochemistry: A high-throughput screen for patterning defects in the mouse cerebellum.</a> J. Histochem. Cytochem. 50, 235-244 (2002).</li><li>Kim, S. -. H., Che, P., Chung, S. -. H., Doorn, D., Hoy, M., Larouche, M., Marzban, H., Sarna, J., Zahedi, S., Hawkes, R. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Whole-Mount+Immunohistochemistry+of+the+Brain.">Whole-Mount Immunohistochemistry of the Brain.</a> Current Protocols in Neuroscience. , (2006).</li><li>Dent, J. A., Polson, A. G., Klymkowsky, M. W. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=A+whole-mount+immunocytochemical+analysis+of+the+expression+of+the+intermediate+filament+protein+vimentin+in+Xenopus.">A whole-mount immunocytochemical analysis of the expression of the intermediate filament protein vimentin in Xenopus.</a> Development. 105, 61-74 (1989).</li><li>Sillitoe, R. V., Malz, C. R., Rockland, K., Hawkes, R. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Antigenic+compartmentation+of+the+primate+and+tree+shrew+cerebellum:+a+common+topography+of+zebrin+II+in+Macaca+mulatta+and+Tupaia+belangeri.">Antigenic compartmentation of the primate and tree shrew cerebellum: a common topography of zebrin II in Macaca mulatta and Tupaia belangeri.</a> J. Anat. 204, 257-269 (2004).</li><li>Ozol, K., Hayden, J. M., Oberdick, J., Hawkes, R. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Transverse+zones+in+the+vermis+of+the+mouse+cerebellum.">Transverse zones in the vermis of the mouse cerebellum.</a> J. Comp. Neurol. 412, 95-111 (1999).</li><li>Apps, R., Hawkes, R. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Cerebellar+cortical+organization:+a+one-map+hypothesis.">Cerebellar cortical organization: a one-map hypothesis.</a> Nat. Rev. Neurosci. 10, 670-681 (2009).</li><li>Reeber, S. L., Sillitoe, R. V. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Patterned+expression+of+a+cocaine-+and+amphetamine-regulated+transcript+peptide+reveals+complex+circuit+topography+in+the+rodent+cerebellar+cortex.">Patterned expression of a cocaine- and amphetamine-regulated transcript peptide reveals complex circuit topography in the rodent cerebellar cortex.</a> J. Comp. Neurol. 519, 1781-1796 (2011).</li><li>Sarna, J. R., Marzban, H., Watanabe, M., Hawkes, R. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Complementary+stripes+of+phospholipase+Cbeta3+and+Cbeta4+expression+by+Purkinje+cell+subsets+in+the+mouse+cerebellum.">Complementary stripes of phospholipase Cbeta3 and Cbeta4 expression by Purkinje cell subsets in the mouse cerebellum.</a> J. Comp. Neurol. 496, 303-313 (2006).</li><li>Demilly, A., Reeber, S. L., Gebre, S. A., Sillitoe, R. V. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Neurofilament+heavy+chain+expression+reveals+a+unique+parasagittal+stripe+topography+in+the+mouse+cerebellum.">Neurofilament heavy chain expression reveals a unique parasagittal stripe topography in the mouse cerebellum.</a> Cerebellum. 10, 409-421 (2011).</li><li>Larouche, M., Hawkes, R. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=From+clusters+to+stripes:+the+developmental+origins+of+adult+cerebellar+compartmentation.">From clusters to stripes: the developmental origins of adult cerebellar compartmentation.</a> Cerebellum. 5, 77-88 (2006).</li><li>Marzban, H., Chung, S., Watanabe, M., Hawkes, R. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Phospholipase+Cbeta4+expression+reveals+the+continuity+of+cerebellar+topography+through+development.">Phospholipase Cbeta4 expression reveals the continuity of cerebellar topography through development.</a> J. Comp. Neurol. 502, 857-871 (2007).</li><li>Blank, M. C., Grinberg, I., Aryee, E., Laliberte, C., Chizhikov, V. V., Henkelman, R. M., Millen, K. J. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Multiple+developmental+programs+are+altered+by+loss+of+Zic1+and+Zic4+to+cause+Dandy-Walker+malformation+cerebellar+pathogenesis.">Multiple developmental programs are altered by loss of Zic1 and Zic4 to cause Dandy-Walker malformation cerebellar pathogenesis.</a> Development. 138, 1207-1216 (2011).</li><li>Sawada, K., Sakata-Haga, H., Fukui, Y. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Alternating+array+of+tyrosine+hydroxylase+and+heat+shock+protein+25+immunopositive+Purkinje+cell+stripes+in+zebrin+II-defined+transverse+zone+of+the+cerebellum+of+rolling+mouse.">Alternating array of tyrosine hydroxylase and heat shock protein 25 immunopositive Purkinje cell stripes in zebrin II-defined transverse zone of the cerebellum of rolling mouse.</a> Nagoya. Brain Res. 1343, 46-53 (2010).</li><li>Sawada, K., Fukui, Y., Hawkes, R. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Spatial+distribution+of+corticotropin-releasing+factor+immunopositive+climbing+fibers+in+the+mouse+cerebellum:+Analysis+by+whole+mount+immunohistochemistry.">Spatial distribution of corticotropin-releasing factor immunopositive climbing fibers in the mouse cerebellum: Analysis by whole mount immunohistochemistry.</a> Brain Res. 1222, 106-117 (2008).</li><li>Marzban, H., Hawkes, R. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=On+the+architecture+of+the+posterior+zone+of+the+cerebellum.">On the architecture of the posterior zone of the cerebellum.</a> Cerebellum. 10, 422-434 (2011).</li><li>Pakan, J. M., Graham, D. J., Wylie, D. R. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Organization+of+visual+mossy+fiber+projections+and+zebrin+expression+in+the+pigeon+vestibulocerebellum.">Organization of visual mossy fiber projections and zebrin expression in the pigeon vestibulocerebellum.</a> J. Comp. Neurol. 518, 175-198 (2010).</li><li>Iwaniuk, A. N., Marzban, H., Pakan, J. M., Watanabe, M., Hawkes, R., Wylie, D. R. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Compartmentation+of+the+cerebellar+cortex+of+hummingbirds+(Aves:+Trochilidae)+revealed+by+the+expression+of+zebrin+II+and+phospholipase+C+beta+4.">Compartmentation of the cerebellar cortex of hummingbirds (Aves: Trochilidae) revealed by the expression of zebrin II and phospholipase C beta 4.</a> J. Chem. Neuroanat. 37, 55-63 (2009).</li><li>Sarna, J. R., Larouche, M., Marzban, H., Sillitoe, R. V., Rancourt, D. E., Hawkes, R. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Patterned+Purkinje+cell+degeneration+in+mouse+models+of+Niemann-Pick+type+C+disease.">Patterned Purkinje cell degeneration in mouse models of Niemann-Pick type C disease.</a> J. Comp. Neurol. 456, 279-291 (2003).</li><li>Sarna, J. R., Hawkes, R. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Patterned+Purkinje+cell+loss+in+the+ataxic+sticky+mouse.">Patterned Purkinje cell loss in the ataxic sticky mouse.</a> Eur. J. Neurosci. 34, 79-86 (2011).</li><li>El-Bizri, N., Guignabert, C., Wang, L., Cheng, A., Stankunas, K., Chang, C. P., Mishina, Y., Rabinovitch, M. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=SM22alpha-targeted+deletion+of+bone+morphogenetic+protein+receptor+1A+in+mice+impairs+cardiac+and+vascular+development,+and+influences+organogenesis.">SM22alpha-targeted deletion of bone morphogenetic protein receptor 1A in mice impairs cardiac and vascular development, and influences organogenesis.</a> Development. 135, 2981-2991 (2008).</li><li>Mondrinos, M. J., Koutzaki, S., Lelkes, P. I., Finck, C. M. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=A+tissue-engineered+model+of+fetal+distal+lung+tissue.">A tissue-engineered model of fetal distal lung tissue.</a> Am. J. Physiol. Lung Cell Mol. Physiol. 293, 639-650 (2007).</li><li>Coppola, E., Rallu, M., Richard, J., Dufour, S., Riethmacher, D., Guillemot, F., Goridis, C., Brunet, J. F. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Epibranchial+ganglia+orchestrate+the+development+of+the+cranial+neurogenic+crest.">Epibranchial ganglia orchestrate the development of the cranial neurogenic crest.</a> Proc. Nat. Acad. Sci. 107, 2066-2071 (2010).</li><li>Kubilus, J. K., Linsenmayer, T. F. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Developmental+guidance+of+embryonic+corneal+innervation:+roles+of+Semaphorin3A+and+Slit2.">Developmental guidance of embryonic corneal innervation: roles of Semaphorin3A and Slit2.</a> Dev. Biol. 344, 172-184 (2010).</li><li>Reeber, S. L., Gebre, S. A., Sillitoe, R. V. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Fluorescence+mapping+of+afferent+topography+in+three+dimensions.">Fluorescence mapping of afferent topography in three dimensions.</a> Brain Struct. Funct. 216, 159-169 (2011).</li><li>Davis, C. A. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Whole-mount+immunohistochemistry.">Whole-mount immunohistochemistry.</a> Methods Enzymol. 225, 502-516 (1993).</li></ol>

Vi har beskrevet de tekniske detaljer, der er nødvendige for en vellykket wholemount farvning ved hjælp af en alsidig immunhistokemisk tilgang til afslørende protein-ekspression i udviklende og voksne hjerne. Ved hjælp af denne fremgangsmåde, kan komplekse molekylære ekspressionsmønstre skal analyseres, og hjernen topografi værdsat uden behov for besværlige og tidskrævende væv koblinger procedurer. Denne protokol er blevet anvendt til at afsløre den mønstrede ekspressionen af ad...

<table border="1"><tbody><tr><td> Materialer </td><td> Funktion i protokol </td></tr><tr><td> Perfusion (Fisher Scientific/13-876-2) </td><td> Giver mulighed for konsekvent og langsom perfusion. </td></tr><tr><td> Sharp-spids saks (FST/14081-08) </td><td> Generel brug i perfusion og dissektion. </td></tr><tr><td> Blunt-tip Tang (FST/91100-12) </td><td> At stabilisere hjertet for indføring af perfusion nålen. </td></tr><tr><td> Tang (FST af Dumont AA/11210-10) </td><td> Til brug ved dissektion af hjernen fra kraniet, og at adskille cerebellum fra resten af ​​hjernen. Disse er vigtige, fordi de har en let afrundet spids, som hjælper med at minimere skade på cerebellum under dissektion. </td></tr><tr><td> Nutator (Fisher Scientific) </td><td> Anvendes til at holde vævet i bevægelse under inkubationsperioder. </td></tr><tr><td> 1,5 ml rør (Sarstedt / Screw Cap Micro Tube) </td><td> Alle trin i sintochemistry protokol finder sted i disse mikrorør. Den afrundede bund sikrer, at cerebellum forbliver i bevægelse. </td></tr><tr><td> Hulske (FST/10370-17) </td><td> Anvendes til at holde wholemounts i mikrorør under forsigtig dekantering ud brugt opløsningen. </td></tr><tr><td> Leica MZ16 FA mikroskop </td><td> Bruges til at undersøge wholemount farvning. </td></tr><tr><td> Leica DFC3000 FX kamera </td><td> Anvendes til at indfange wholemount billeder. </td></tr></tbody></table> Tabel 1. <table border="1"><tbody><tr><td colspan="9"> Eksempel kalender for et typisk wholemount eksperiment </td></tr><tr><td> Dag 1 </td><td colspan="4"> Dent har fix, stuetemperatur, 8 timer </td><td colspan="4"> Tens blegemiddel, 4 ° C natten over </td></tr><tr><td> Dag 2 </td><td colspan="3"> 100% MeOH, stuetemperatur, 2x, 30 minutter hver </td><td colspan="3"> 100% MeOH, frost / tø, 4x, 30 min/15 min </td><td colspa<html> n = &quot;2&quot;&gt; 100% MeOH, -80 ° C natten over </td></tr><tr><td> Dag 3 </td><td> 50% MeOH/50% PBS, stuetemperatur, 60-90 min </td><td colspan="2"> 15% MeOH / 85% PBS, stuetemperatur, 60-90 min </td><td colspan="2"> 100% PBS, stuetemperatur, 60-90 min </td><td> 10μg/mL proteinase K i PBS, stuetemperatur, 2-3 min </td><td> 100% PBS, stuetemperatur, 3x, 10 minutter hver </td><td> PMT, 4 ° C natten over </td></tr><tr><td> Dag 4-5 </td><td colspan="8"> PMT + 1 ° antistof + 5% DMSO, 4 ° C, 48 timer </td></tr><tr><td> Dag 6 </td><td colspan="3"> PMT, 4 ° C, 2-3x, 2-3 timer hver </td><td colspan="5"> PMT + 2 ° antistof + 5% DMSO, 4 ° C, 24 timer (eller begynde amplifikationstrinnene med ABC-kompleks) </td></tr><tr><td> Dag 7 </td><td colspan="2"> PMT, 4 ° C, 2-3x, 2-3 timer hver </td><td colspan="3"> PBT, stuetemperatur, 2 timer </td><td colspan="3"> Inkubere i frisk DAB i PBS indtil optimal farvning visualiseres </td></tr></tbody></table>_content &quot;&gt; Tabel 2. <table border="1"><tbody><tr><td colspan="2"> Opskrifter (* = fremstille frisk hver gang) </td></tr><tr><td> PBS (phosphatpufret saltvand) </td><td> 0,1 M phosphatbufret saltvand i deioniseret vand. pH 7,2 (Sigma tabletter, P4417) </td></tr><tr><td> PFA (paraformaldehyd) </td><td> Fremstilles og opbevares frosset i en 20% opløsning og derefter fortyndet til 4% i PBS i arbejdsopløsningen (Fisher Scientific, T353) </td></tr><tr><td> Dent har Fixativ 3 * </td><td> 4 dele methanol 1 del dimethylsulfoxid (DMSO, Fisher Scientific, D159-4) </td></tr><tr><td> Dent har Bleach 3 * </td><td> 4 dele methanol 1 del dimethylsulfoxid (DMSO, Fisher Scientific, D159-4) 1 del 30% hydrogenperoxid </td></tr><tr><td> Enzymatisk nedbrydning </td><td> 10 ug / ml proteinase K (Roche Diagnostics, 03115828001) i PBS. </td></tr><tr><td> PBST </td><td> PBS indeholdende: 0,1% Tween-20 (Fisher Scientific, BP337, Triton kan også anvendes i stedet for Tween-20 i alle tilfælde). </td></tr><tr><td> PMT 25 * </td><td> PBS indeholdende: 2% ikke-fedtholdig skummetmælkspulver (Carnation foretrækkes) 0,1% Tween-20 (Fisher Scientific, BP337) </td></tr><tr><td> PBT 25 * </td><td> PBS indeholdende: 0,2% bovint serumalbumin (Sigma; B9001S) 0,1% Tween-20 (Fisher Scientific, BP337) </td></tr><tr><td> DAB * </td><td> Opløs en 10 mg tablet af 3,3-diaminobenzidin (Sigma-Aldrich, D5905) i 40 ml PBS. Tilsæt 10 gl 30% hydrogenperoxid for at initiere reaktionen). </td></tr><tr><td> ABC kompleksopløsning </td><td> Vectastain-kit (Vector Laboratories, Inc.; PK-4000) </td></tr></tbody></table> Tabel 3.

wholemount immunohistochemistry for revealing complex brain topography

Den gentagne og godt forstået cellulære arkitektur af lillehjernen gør det til en ideel model for at udforske hjernen topografi. Underliggende dets relativt ensartet cytoarchitecture er en kompleks række af parasagittal domæner genet og proteinekspression. Den molekylære opdeling af lillehjernen er afspejlet i den anatomiske og funktionelle organisering af afferente fibre. For fuldt ud at forstå kompleksiteten af ​​cerebellare organisation, vi tidligere har forfinet en wholemount farvning tilgang til high throughput analyse af mønstergivende fejl i musen lillehjernen. Denne protokol beskriver i detaljer de reagenser, værktøjer og praktiske skridt, som er nyttige at kunne afsløre protein ekspressionsmønstre i den voksne mus lillehjernen ved hjælp wholemount immunfarvning. Trinene fremhævet her demonstrere anvendeligheden af ​​denne metode at bruge udtryk zebrinII / aldolaseC som et eksempel på, hvordan den fine topografi i hjernen, kan blive afsløret i sinnative tredimensionelle konformation. Også beskrevet er tilpasninger til den protokol, der giver mulighed for visualisering af protein udtryk i afferente fremskrivninger og store cerebella for sammenlignende studier af molekylære topografi. For at illustrere disse ansøgninger, er data fra afferent farvning af rottecerebellum inkluderet.

Watch this Scientific Journal Video about Wholemount Immunohistochemistry for Revealing Complex Brain Topography at JoVE.com

Wholemount Immunohistochemistry for Revealing Complex Brain Topography

Neurale kredsløb topografisk organiseret i funktionelle rum med specifikke molekylære profiler. Her giver vi de praktiske og tekniske skridt til at afsløre den globale hjerne topografi med et alsidigt wholemount immunhistokemisk farvning tilgang. Vi demonstrerer anvendeligheden af ​​metoden under anvendelse af velforståede cytoarchitecture og kredsløb cerebellum.

Wholemount Immunhistokemi for at afsløre Complex Brain Topografi

The repeated and well-understood cellular architecture of the cerebellum make it an ideal model system for exploring brain topography. Underlying its ...

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