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DOI :
10.3791/4057-v
November 12th, 2012
Chapters
0:05
Title
1:47
Construction of Gene-specific SPA-tagging in E. Coli DY330 Strain
3:54
Culturing and Sonication
5:26
Affinity Purification
7:53
Proteolysis and Sample Preparation for Mass Spectrometry
9:38
Protein Identification by LTQ Orbitap Velos Mass Spectrometer
11:02
Results
14:27
Conclusion
マススペクトロメトリー(APMS)との組み合わせでタグ付けされたタンパク質のアフィニティー精製は、タンパク質相互作用ネットワークの体系的マッピングのため、生物学的プロセスの基本メカニズムを調査するための強力な方法です。ここでは、細菌のために開発最適化された順次ペプチドアフィニティー(SPA)をAPMSの手順を説明大腸菌。
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