Name: imaging glioma initiation in vivo through a polished and reinforced thin-skull cranial window
Uploaded: 2012-11-20T12:00:00
Description: Watch this Scientific Journal Video about Imaging Glioma Initiation In Vivo Through a Polished and Reinforced Thin-skull Cranial Window at JoVE.com

Dette arbejde understøttes af The Jackson Laboratory Cancer Center Pilot Grant og The Maine Cancer Foundation.

1. Protokol <ol><li> Bedøve mus med ketamin og xylazin i en dosis på 0,1 mg ketamin og 0,01 mg xylazin pr 1 g legemsvægt. Carprofen (0,005 mg per 1 g legemsvægt) anvendes til analgesi og administreres præoperativt. </li><li> Alle de kirurgiske værktøjer, herunder den dentale bor, er vanddamp steriliseret i en autoklave. Hvis batch operationer skal udføres, skal spidserne af kirurgiske instrumenter resteriliseres med en glasperle sterilisator (Fine Science Tools FST 250) før hver efterfølgende operation. </li...

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Nøglen til en vellykket porte kraniel vindue er udtynding og polering. Det oprindelige udtynding kan udføres hurtigt, bør der udvises omhu for at sikre en ensartet udtynding af kraniet over et stort område. Vi typisk anvende et tyndt lag saltvand til kraniet, og derefter tynde kraniet en passage ad gangen, således at saltopløsningen fordamper kort efter microdrill har passeret over kraniet gang. Dette giver os mulighed for langsomt, men homogent yderligere tynd kraniet. En rolig hånd under mikroskopet er også n?...

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imaging glioma initiation in vivo through a polished and reinforced thin-skull cranial window

Gliom er en af ​​de mest dødelige former for human cancer. Den mest effektive gliom terapi til dato-kirurgi efterfulgt af strålebehandling-tilbyde patienterne kun beskedne fordele, da de fleste patienter ikke overlever mere end fem år efter diagnosen på grund af gliom tilbagefald 1,2. Opdagelsen af cancer stamceller i humane hjernetumorer det lover godt for at have en enorm indflydelse på udviklingen af nye terapeutiske strategier for gliom 3. Cancerstamceller er defineret ved deres evne til både selv forny og til at differentiere, og menes at være de eneste celler i en tumor, der har kapacitet til at initiere nye tumorer 4. Gliom tilbagefald efter strålebehandling menes at hidrøre fra modstand glioma stamceller (GSCS) til behandling 5-10. In vivo er GSCS vist at opholde sig i en perivaskulær niche, der er vigtig for at bevare deres stamcelle-lignende egenskaber 11-14 . Centralt i organining af GSC niche er vaskulære endothelceller 12. Eksisterende tyder på, at GSCS og deres samspil med de vaskulære endothelceller er vigtige for tumor udvikling, og identificere GSCS og deres samspil med endotelceller som vigtige terapeutiske mål for gliom. Tilstedeværelsen af GSCS bestemmes eksperimentelt ved deres evne til at igangsætte nye svulster på orthotopisk transplantation 15. Dette opnås typisk ved at injicere et bestemt antal GBM celler isoleret fra humane tumorer i hjernen på alvorligt immun-deficiente mus, eller af muse-GBM-celler ind i hjernen på kongene værtsmus. Analyser for tumorvækst udføres derefter efter tilstrækkelig tid til at tillade GSCS blandt de injicerede GBM cellerne give anledning til nye tumorer, typisk flere uger eller måneder. Derfor behøver eksisterende analyser ikke kunne undersøges, om den vigtige patologiske proces af tumorinitiering fra enkelte GSCS in vivo. Derfor væsentligt insights i de specifikke roller GSCS og deres samspil med de vaskulære endotelceller i de tidlige stadier af tumor initiering mangler. Sådanne indsigter er afgørende for udvikling af nye terapeutiske strategier for gliom, og vil få store konsekvenser for forebyggelse af gliom tilbagefald hos patienter. Her har vi tilpasset portene craniale vindue procedure 16 og in vivo to-foton mikroskopi for at tillade visualisering af tumorinitiering fra injicerede GBM celler i hjernen af en levende mus. Vores teknik vil bane vejen for fremtidige indsats for at belyse de centrale signaleringsmekanismer mellem GSCS og vaskulære endotelceller i løbet af gliom indvielse.

Watch this Scientific Journal Video about Imaging Glioma Initiation In Vivo Through a Polished and Reinforced Thin-skull Cranial Window at JoVE.com

Imaging Glioma Initiation In Vivo Through a Polished and Reinforced Thin-skull Cranial Window

Ved at kombinere en poleret og forstærket tynd-skull (havne) kraniel vindue og glioblastom (GBM) celleinjektion, kan vi observere glioma initiering og vækst fra injicerede GBM celler i hjernen hos en levende mus i længderetningen.

Imaging gliom Indledning<em&gt; In Vivo</em&gt; Gennem en poleret og Reinforced Thin-kranium Cranial Window

Glioma is the one of the most lethal forms of human cancer. The most effective glioma therapy to date-surgery followed by radiation treatment-offers ...

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Imaging Glioma Initiation In Vivo Through a Polished and Reinforced Thin-skull Cranial Window

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