Name: imaging glioma initiation in vivo through a polished and reinforced thin-skull cranial window
Uploaded: 2012-11-20T12:00:00
Description: Watch this Scientific Journal Video about Imaging Glioma Initiation In Vivo Through a Polished and Reinforced Thin-skull Cranial Window at JoVE.com

यह काम जैक्सन प्रयोगशाला कैंसर केंद्र पायलट अनुदान और मेन कैंसर फाउंडेशन द्वारा समर्थित है.

1. प्रोटोकॉल <ol><li> और 0.1 मिलीग्राम ketamine और शरीर के वजन के 1 ग्राम प्रति xylazine की 0.01 मिलीग्राम की एक खुराक पर ketamine xylazine साथ माउस anesthetize. Carprofen (0.005 मिलीग्राम शरीर के वजन के 1 ग्राम प्रति) analgesia के लिए किया जाता है और preoperatively प्रशास...

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	<li>Paulino, A. C., Teh, B. S. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Treatment+of+brain+tumors.">Treatment of brain tumors.</a> N. Engl. J. Med. 352, 2350-2353 (2005).</li><li>Stupp, R., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Radiotherapy+plus+concomitant+and+adjuvant+temozolomide+for+glioblastoma.">Radiotherapy plus concomitant and adjuvant temozolomide for glioblastoma.</a> N. Engl. J. Med. 352, 987-996 (2005).</li><li>Singh, S. K., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Identification+of+human+brain+tumour+initiating+cells.">Identification of human brain tumour initiating cells.</a> Nature. 432, 396-401 (2004).</li><li>Hanahan, D., Weinberg, R. 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एक सफल बंदरगाहों कपाल खिड़की कुंजी thinning और चमकाने है. जबकि thinning प्रारंभिक जल्दी से किया जा सकता है, देखभाल सुनिश्चित करने के एक बड़े क्षेत्र में समरूप खोपड़ी के thinning लिया जाना चाहिए. हम आम तौर पर खारा की खोप?...

<table border="1"><tr><td> अभिकर्मक के नाम </td><td> कंपनी </td><td> सूचीपत्र संख्या </td><td> टिप्पणियां (वैकल्पिक) </td></tr><tr><td> सोडियम पाइरूवेट साथ DMEM/F12 (1:1) </td><td> थर्मो विज्ञान Hyclone </td><td> SH3026101 </td><td></td></tr><tr><td> B-27 सीरम मुक्त अनुपूरक (50x) </td><td> Invitrogen </td><td> 17504-044 </td><td></td></tr><tr><td> GlutaMax-1 अनुपूरक </td><td> Invitrogen </td><td> 35050061 </td><td></td></tr><tr><td> पेनिसिलिन - स्ट्रेप्टोमाइसिन समाधान </td><td> थर्मो विज्ञान Hyclone </td><td> SV30010 </td><td></td></tr><tr><td> Accutase एनजाइम सेल टुकड़ी मध्यम </td><td> eBiosciences </td><td> 00-4555-56 </td><td></td></tr><tr><td> T25 बोतल वेंट टोपी हरे रंग </td><td> SARSTEDT </td><td> 83.1810.502 </td><td></td></tr><tr><td> 70% शराब </td><td> JAX LAHS </td><td></td><td></td></tr><tr><td> 10% povidone आयोडीन सामयिक समाधान </td><td> JAX LAHS </td><td></td><td></td></tr><tr><td> Ketamine एचसीएल </td><td> बटलर पशु स्वास्थ्य की आपूर्ति </td><td> 11695-0550-1 # एनडीसी </td><td></td></tr><tr><td> Xylazine </td><td> Akorn, Inc </td><td> 139-236 # नाडा </td><td></td></tr><tr><td> Carprofen </td><td> JAX LAHS </td><td></td><td></td></tr><tr><td> नेत्र मरहम </td><td> Dechra पशु उत्पाद </td><td> 17033-211-38 </td><td></td></tr><tr><td> 0.5% lidocaine एचसीएल </td><td> Regent इंक, </td><td> 0517-0625-25 एनडीसी </td><td></td></tr><tr><td> Cyanoacrylate गोंद </td><td> हेंकेल कार्पोरेशन </td><td> 46551 </td><td></td></tr><tr><td> बाँझ swabs </td><td> फिशर साइंटिफिक </td><td> 23-400-114 </td><td></td></tr><tr><td> हीरे का पेस्ट </td><td> विजेट आपूर्ति </td><td> BBE60 </td><td></td></tr><tr><td> टिन ऑक्साइड </td><td> LORTONE इंक, </td><td> 591-038 </td><td></td></tr><tr><td> लाइनर बॉण्ड 2V </td><td> Kuraray मेडिकल इंक </td><td> +१,९२१-के.ए. </td><td></td></tr><tr><td> AP-X Clearfil </td><td> Kuraray मेडिकल इंक </td><td> 1721-के.ए. </td><td></td></tr><tr><td> खारा </td><td> JAX LAHS </td><td></td><td></td></tr><tr><td> कांच के कवर </td><td> वार्नर उपकरण </td><td> 64-0720 </td><td></td></tr><tr><td> हेक्स अखरोट </td><td> छोटे भागों, Inc </td><td> HNX 0,090 - सी </td><td></td></tr><tr><td> सिरिंज पंप </td><td> Syringepump.com </td><td> NE-1000 </td><td></td></tr><tr><td> दो photon इमेजिंग प्रणाली </td><td> कस्टम निर्मित (कोई वाणिज्यिक प्रणालीकाम) </td><td></td><td></td></tr></table>

imaging glioma initiation in vivo through a polished and reinforced thin-skull cranial window

Glioma is the one of the most lethal forms of human cancer. The most effective glioma therapy to date-surgery followed by radiation treatment-offers patients only modest benefits, as most patients do not survive more than five years following diagnosis due to glioma relapse 1,2. The discovery of cancer stem cells in human brain tumors holds promise for having an enormous impact on the development of novel therapeutic strategies for glioma 3. Cancer stem cells are defined by their ability both to self-renew and to differentiate, and are thought to be the only cells in a tumor that have the capacity to initiate new tumors 4. Glioma relapse following radiation therapy is thought to arise from resistance of glioma stem cells (GSCs) to therapy 5-10. In vivo, GSCs are shown to reside in a perivascular niche that is important for maintaining their stem cell-like characteristics 11-14. Central to the organization of the GSC niche are vascular endothelial cells 12. Existing evidence suggests that GSCs and their interaction with the vascular endothelial cells are important for tumor development, and identify GSCs and their interaction with endothelial cells as important therapeutic targets for glioma. The presence of GSCs is determined experimentally by their capability to initiate new tumors upon orthotopic transplantation 15. This is typically achieved by injecting a specific number of GBM cells isolated from human tumors into the brains of severely immuno-deficient mice, or of mouse GBM cells into the brains of congenic host mice. Assays for tumor growth are then performed following sufficient time to allow GSCs among the injected GBM cells to give rise to new tumors-typically several weeks or months. Hence, existing assays do not allow examination of the important pathological process of tumor initiation from single GSCs in vivo. Consequently, essential insights into the specific roles of GSCs and their interaction with the vascular endothelial cells in the early stages of tumor initiation are lacking. Such insights are critical for developing novel therapeutic strategies for glioma, and will have great implications for preventing glioma relapse in patients. Here we have adapted the PoRTS cranial window procedure 16and in vivo two-photon microscopy to allow visualization of tumor initiation from injected GBM cells in the brain of a live mouse. Our technique will pave the way for future efforts to elucidate the key signaling mechanisms between GSCs and vascular endothelial cells during glioma initiation.

Watch this Scientific Journal Video about Imaging Glioma Initiation In Vivo Through a Polished and Reinforced Thin-skull Cranial Window at JoVE.com

Imaging Glioma Initiation In Vivo Through a Polished and Reinforced Thin-skull Cranial Window

एक पॉलिश और प्रबलित (बंदरगाह) पतली खोपड़ी कपाल खिड़की और glioblastoma (जीबीएम) सेल इंजेक्शन के संयोजन से, हम glioma दीक्षा और इंजेक्शन जीबीएम कोशिकाओं से एक जीवित longitudinally माउस के मस्तिष्क में वृद्धि का पालन कर सकते हैं.

इमेजिंग Glioma दीक्षा<em&gt; Vivo में</em&gt; एक पॉलिश और प्रबलित पतली खोपड़ी कपाल खिड़की के माध्यम से

Glioma is the one of the most lethal forms of human cancer. The most effective glioma therapy to date-surgery followed by radiation treatment-offers ...

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Imaging Glioma Initiation In Vivo Through a Polished and Reinforced Thin-skull Cranial Window

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एक कपाल विंडो का उपयोग करने के लिए endothelin-1 प्रेरित मध्य सेरेब्रल धमनी रोड़ा के दौरान मध्यम प्रमस्तिष्क धमनी कल्पना

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3 डी Neuronavigation<em&gt; में Vivo</em&gt; एक सहज माइग्रेन का सिरदर्द के दौरान एक मरीज की मस्तिष्क के माध्यम से

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