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Rapid and Efficient Generation of Neurons from Human Pluripotent Stem Cells in a Multititre Plate Format

DOI :

10.3791/4335-v

March 5th, 2013

March 5th, 2013

43,427 Views

1Max Planck Institute for Molecular Biomedicine, 2Medical Faculty, University of Münster

Protocols for neuronal differentiation of pluripotent human stem cells (hPSCs) are often time-consuming and require substantial cell culture skills. Here, we have adapted a small molecule-based differentiation procedure to a multititre plate format, allowing simple, rapid, and efficient generation of human neurons in a controlled manner.

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