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Reprogramming Human Somatic Cells into Induced Pluripotent Stem Cells (iPSCs) Using Retroviral Vector with GFP

DOI :

10.3791/3804-v

April 3rd, 2012

April 3rd, 2012

20,135 Views

1Yale Stem Cell Center, Department of Genetics, Yale School of Medicine

A method to generate human induced pluripotent stem cells (iPSCs) via retrovirus-mediated ectopic expression of OCT4, SOX2, KLF4 and MYC is described. A practical way to identify human iPSC colonies based on GFP expression is also discussed.

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Reprogramming

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