JoVE Logo
Faculty Resource Center

Sign In

Performing Vaginal Lavage, Crystal Violet Staining, and Vaginal Cytological Evaluation for Mouse Estrous Cycle Staging Identification

DOI :

10.3791/4389-v

9:01 min

September 15th, 2012

September 15th, 2012

104,709 Views

1Department of Biochemistry, Microbiology and Immunology, Neural Regeneration Laboratory and Ottawa Institute of Systems Biology, 2Department of Cellular and Molecular Medicine, University of Ottawa , 3CIHR Program in Neurodegenerative Lipidomics, University of Ottawa , 4Carleton Immersive Media Studio, Azrieli School of Architecture and Urbanism

Here, we describe how to identify the stage of the murine reproductive (proestrus, estrus, metestrus, or diestrus) by simple, non-invasive collection and cytological assessment of vaginal smear samples. We further describe how vaginal cytology reflects circulating hormonal levels underlying transition through the murine reproductive cycle.

Tags

Vaginal Lavage

-- Views

Related Videos

article

A Reversible, Non-invasive Method for Airway Resistance Measurements and Bronchoalveolar Lavage Fluid Sampling in Mice

article

In vivo Imaging and Therapeutic Treatments in an Orthotopic Mouse Model of Ovarian Cancer

article

Evaluation of Mammary Gland Development and Function in Mouse Models

article

Live Cell Cycle Analysis of Drosophila Tissues using the Attune Acoustic Focusing Cytometer and Vybrant DyeCycle Violet DNA Stain

article

Pyrosequencing for Microbial Identification and Characterization

article

Cytological Analysis of Spermatogenesis: Live and Fixed Preparations of Drosophila Testes

article

Methods for Staging Pupal Periods and Measurement of Wing Pigmentation of Drosophila guttifera

article

Trans-inner Cell Mass Injection of Embryonic Stem Cells Leads to Higher Chimerism Rates

article

Quantification of Lipid Abundance and Evaluation of Lipid Distribution in Caenorhabditis elegans by Nile Red and Oil Red O Staining

article

Identification and Dissection of Diverse Mouse Adipose Depots

JoVE Logo

Privacy

Terms of Use

Policies

Research

Education

ABOUT JoVE

Copyright © 2024 MyJoVE Corporation. All rights reserved