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Quantitative FRET (Förster Resonance Energy Transfer) Analysis for SENP1 Protease Kinetics Determination

18.9K Views

16:02 min

February 21st, 2013

DOI :

10.3791/4430-v

February 21st, 2013


Transcript

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Quantitative FRET Analysis

Chapters in this video

0:05

Title

2:20

Plasmid Constructs

3:07

Protein Expression and Purification

6:16

FRET-based Protease Assay for Enzyme Kinetic Study

8:54

Quantitative FRET Spectrum Analysis

13:36

Representative Results

15:11

Conclusion

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