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Use of Recombinant Fusion Proteins in a Fluorescent Protease Assay Platform and Their In-gel Renaturation

DOI :

10.3791/58824-v

19:23 min

January 16th, 2019

January 16th, 2019

8,838 Views

1Department of Biochemistry and Molecular Biology, Faculty of Medicine, University of Debrecen, 2Biotechnology Research Department, Gedeon Richter Plc

Here, we present the detailed procedure of a recently developed protease assay platform utilizing N-terminal hexahistidine/maltose-binding protein and fluorescent protein-fused recombinant substrates attached to the surface of nickel-nitrilotriacetic acid magnetic agarose beads. A subsequent in-gel analysis of the assay samples separated by sodium dodecyl sulfate-polyacrylamide gel electrophoresis is also presented.

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Recombinant Fusion Proteins

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