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A High-throughput Method for Measurement of Glomerular Filtration Rate in Conscious Mice

DOI :

10.3791/50330-v

7:07 min

May 10th, 2013

May 10th, 2013

42,094 Views

1Division of Nephrology-Hypertension, Department of Medicine, University of California, San Diego , 2San Diego VA Healthcare System

Measurement of glomerular filtration rate (GFR) is the gold standard for kidney function assessment. Here we describe a high-throughput method which allows the determination of GFR in conscious mice by using a single bolus injection, determination of fluorescein isothiocyanate (FITC)-inulin in plasma and calculation of GFR by a two-phase exponential decay model.

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High throughput

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