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Measurement and Analysis of Extracellular Acid Production to Determine Glycolytic Rate

DOI :

10.3791/53464-v

6:47 min

December 12th, 2015

December 12th, 2015

24,458 Views

1Touro University California College of Pharmacy, 2Buck Institute for Research on Aging

Glycolysis is a defining metabolic marker in multiple biological systems. Monitoring glycolysis by measuring the extracellular flux of H+ is common, but requires correction to be quantitative and unambiguous. Here, we demonstrate how to gather and correct extracellular flux data to distinguish between respiratory and glycolytic sources of extracellular acidification.

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Measurement

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