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High-throughput Crystallization of Membrane Proteins Using the Lipidic Bicelle Method

DOI :

10.3791/3383-v

January 9th, 2012

January 9th, 2012

23,406 Views

1UCLA-DOE Institute for Genomics and Proteomics, University of California Los Angeles , 2Department of Physiology, David Geffen School of Medicine, UCLA

Bicelles are lipid/amphiphile mixtures that maintain membrane proteins (MPs) within a lipid bilayer but have unique phase behavior that facilitates high-throughput screening by crystallization robots. This technique has successfully produced a number of high-resolution structures from both prokaryotic and eukaryotic sources. This video describes protocols for generating the lipidic bicelle mixture, incorporating MPs into the bicelle mixture, setting up crystallizations trials (manually as well as robotically) and harvest

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High throughput Crystallization

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