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Three-dimensional Confocal Analysis of Microglia/macrophage Markers of Polarization in Experimental Brain Injury

DOI :

10.3791/50605-v

September 4th, 2013

September 4th, 2013

11,399 Views

1Department of Neuroscience, IRCCS - Istituto di Ricerche Farmacologiche Mario Negri

A way to gain new insights into the complexity of the brain inflammatory response is presented. We describe immunofluorescence-based protocols followed by three-dimensional confocal analysis to investigate the pattern of co-expression of microglia/macrophage phenotype markers in a mouse model of focal ischemia.

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Keywords 3D Confocal Analysis

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