JoVE Logo
Faculty Resource Center

Sign In

Concurrent Quantification of Cellular and Extracellular Components of Biofilms

DOI :

10.3791/50639-v

10:18 min

December 10th, 2013

December 10th, 2013

8,030 Views

1Department of Dental Materials, College of Dentistry, University of Oklahoma Health Sciences Center, 2Department of Biostatistics and Epidemiology, College of Public Health, University of Oklahoma Health Sciences Center, 3Department of Biological Sciences, School of Mathematics and Natural Sciences, The Copperbelt University

A protocol for the concurrent quantification and comparison of three cellular and extracellular components within biofilms is presented. The methodology involves the use of confocal laser scanning microscopy, biofilm structural analysis and visualization software, and statistical analysis software.

Tags

Biofilms

-- Views

Related Videos

article

Neutrophil Extracellular Traps: How to Generate and Visualize Them

article

Visualisation and Quantification of Intracellular Interactions of Neisseria meningitidis and Human α-actinin by Confocal Imaging

article

Co-culture Models of Pseudomonas aeruginosa Biofilms Grown on Live Human Airway Cells

article

Enzyme-linked Immunospot Assay (ELISPOT): Quantification of Th-1 Cellular Immune Responses Against Microbial Antigens

article

Growth of Mycobacterium tuberculosis Biofilms

article

Anti-virulent Disruption of Pathogenic Biofilms using Engineered Quorum-quenching Lactonases

article

Ratiometric Imaging of Extracellular pH in Dental Biofilms

article

A Simple Fluorescence Assay for Quantification of Canine Neutrophil Extracellular Trap Release

article

Paraffin Embedding and Thin Sectioning of Microbial Colony Biofilms for Microscopic Analysis

article

Automated Image-Based Quantification of Neutrophil Extracellular Traps Using NETQUANT

JoVE Logo

Privacy

Terms of Use

Policies

Research

Education

ABOUT JoVE

Copyright © 2024 MyJoVE Corporation. All rights reserved