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Single-cell Analysis of Bacillus subtilis Biofilms Using Fluorescence Microscopy and Flow Cytometry

DOI :

10.3791/3796-v

February 15th, 2012

February 15th, 2012

20,027 Views

1Institute for Molecular Infection Biology (IMIB), University of Würzburg

Microbial biofilms are generally constituted by distinct subpopulations of specialized cells. Single-cell analysis of these subpopulations requires the use of fluorescent reporters. Here we describe a protocol to visualize and monitor several subpopulationswithin B. subtilis biofilms using fluorescence microscopy and flow cytometry.

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