JoVE Logo
Faculty Resource Center

Sign In

Microarray-based Identification of Individual HERV Loci Expression: Application to Biomarker Discovery in Prostate Cancer (Video) | JoVE

DOI :

10.3791/50713-v

13:19 min

November 2nd, 2013

November 2nd, 2013

16,144 Views

1Joint Unit Hospices de Lyon-bioMérieux, 2Medical Diagnostic Discovery Department, BioMérieux, 3Department of Pathology and Cytology, Centre Hospitalier Lyon Sud, Hospices Civils de Lyon, 4Medical Faculty, Lyon 1 University, 5Data and Knowledge Laboratory, BioMérieux, 6Department of Biochemistry and Molecular Biology, Centre Hospitalier Lyon Sud, Hospices Civils de Lyon, 7Department of Urology, Centre Hospitalier Lyon Sud, Hospices Civils de Lyon

Human endogenous retroviruses (HERV), which occupy 8% of the human genome, retain scarce coding capacities but a hundred thousand long terminal repeats (LTRs). A custom Affymetrix microarray was designed to identify individual HERV locus expression and was used on prostate cancer tissues as a proof of concept for future clinical studies.

Tags

Microarray

-- Views

Related Videos

article

Evaluation of Nanoparticle Uptake in Tumors in Real Time Using Intravital Imaging (Video) | JoVE

article

The Use of Reverse Phase Protein Arrays (RPPA) to Explore Protein Expression Variation within Individual Renal Cell Cancers (Video) | JoVE

article

A Novel High-resolution In vivo Imaging Technique to Study the Dynamic Response of Intracranial Structures to Tumor Growth and Therapeutics (Video) | JoVE

article

An Orthotopic Murine Model of Human Prostate Cancer Metastasis (Video) | JoVE

article

Isolation of Cancer Stem Cells From Human Prostate Cancer Samples (Video) | JoVE

article

MiRNA Expression Analyses in Prostate Cancer Clinical Tissues Video (Video) | JoVE

article

Generation of Prostate Cancer Patient Derived Xenograft Models from Circulating Tumor Cells Video (Video) | JoVE

article

Clinicopathological Analysis of miRNA Expression in Breast Cancer Tissues by Using miRNA In Situ Hybridization Video (Video) | JoVE

article

Pre-clinical Orthotopic Murine Model of Human Prostate Cancer Video (Video) | JoVE

article

Precision Implementation of Minimal Erythema Dose MED Testing to Assess Individual Variation in Human Inflammatory Response Video (Video) | JoVE

JoVE Logo

Privacy

Terms of Use

Policies

Research

Education

ABOUT JoVE

Copyright © 2024 MyJoVE Corporation. All rights reserved