Microarray-based Identification of Individual HERV Loci Expression: Application to Biomarker Discovery in Prostate Cancer

16.4K Views

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13:19 min

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November 2nd, 2013

DOI :

10.3791/50713-v

November 2nd, 2013

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Philippe Pérot¹^,², Valérie Cheynet¹^,², Myriam Decaussin-Petrucci¹^,³^,⁴, Guy Oriol¹^,², Nathalie Mugnier⁵, Claire Rodriguez-Lafrasse¹^,⁴^,⁶, Alain Ruffion¹^,⁴^,⁷, François Mallet¹^,²

¹Joint Unit Hospices de Lyon-bioMérieux, ²Medical Diagnostic Discovery Department, BioMérieux, ³Department of Pathology and Cytology, Centre Hospitalier Lyon Sud, Hospices Civils de Lyon, ⁴Medical Faculty, Lyon 1 University, ⁵Data and Knowledge Laboratory, BioMérieux, ⁶Department of Biochemistry and Molecular Biology, Centre Hospitalier Lyon Sud, Hospices Civils de Lyon, ⁷Department of Urology, Centre Hospitalier Lyon Sud, Hospices Civils de Lyon

Summary

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Transcriptional Regulation

Genome

Chapters in this video

0:05

Title

2:42

Handling of Prostrate Tissues

4:03

RNA Extraction, Purification, and Quality Control

5:53

Target Preparation

7:33

Hybridization to the HERV Chip Microarray

9:24

Scanning and Data Analysis

10:14

Results: Identification of Active HERV Loci in Prostrate Tissues

11:49

Conclusion

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