Name: a new murine model of endovascular aortic aneurysm repair
Uploaded: 2013-07-07T14:00:00
Description: Watch this Scientific Journal Video about A New Murine Model of Endovascular Aortic Aneurysm Repair at JoVE.com

Forfatterne vil gerne takke Christine Guesdon (Abbott) for de gaver de Stentgrafts.

Metoder Protokollen er blevet godkendt af Bichat-Debré Etik udvalg (N ° 2012-15/698-0074). Den elastase perfusion model er første gang beskrevet af Anidjar i 1990 1: abdominalt aortaaneurisme er foranlediget af elastase perfusion i Wistar rotter i alderen 8 til 9 uger 250-300 g. To til fire uger senere, er en re-laparotomi udføres, og en diameter på 3 mm dækket koronar stentgraft bruges til at udelukke aneurismen. Transplantatet, indsættes gennem e...

<ol>
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M., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=The+significance+of+endoleaks+in+thoracic+endovascular+aneurysm+repair.">The significance of endoleaks in thoracic endovascular aneurysm repair.</a> Ann. Vasc. Surg. 25, 345-351 (2011).</li><li>Buth, J., Harris, P. L., van Marrewijk, C., Fransen, G. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=The+significance+and+management+of+different+types+of+endoleaks.">The significance and management of different types of endoleaks.</a> Semin. Vasc. Surg. 16, 95-102 (2003).</li><li>Fransen, G. A., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Rupture+of+infra-renal+aortic+aneurysm+after+endovascular+repair:+a+series+from+EUROSTAR+registry.">Rupture of infra-renal aortic aneurysm after endovascular repair: a series from EUROSTAR registry.</a> Eur. J. Vasc. Endovasc. Surg. 26, 487-493 (2003).</li><li>van der Vaart, M. G., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Application+of+PET/SPECT+imaging+in+vascular+disease.">Application of PET/SPECT imaging in vascular disease.</a> Eur. J. Vasc. Endovasc. 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Surg. 19, 446-456 (1994).</li><li>Gawenda, M., Jaschke, G., Winter, S., Wassmer, G., Brunkwall, J. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Endotension+as+a+result+of+pressure+transmission+through+the+graft+following+endovascular+aneurysm+repair--an+in+vitro+study.">Endotension as a result of pressure transmission through the graft following endovascular aneurysm repair--an in vitro study.</a> Eur. J. Vasc. Endovasc. Surg. 26, 501-505 (2003).</li><li>Delbosc, S., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Porphyromonas+gingivalis+Participates+in+Pathogenesis+of+Human+Abdominal+Aortic+Aneurysm+by+Neutrophil+Activation.+Proof+of+Concept+in+Rats.">Porphyromonas gingivalis Participates in Pathogenesis of Human Abdominal Aortic Aneurysm by Neutrophil Activation. Proof of Concept in Rats.</a> PLoS One. 6, e18679 (2011).</li><li>Trollope, A., Moxon, J. V., Moran, C. S., Golledge, J. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Animal+models+of+abdominal+aortic+aneurysm+and+their+role+in+furthering+management+of+human+disease.">Animal models of abdominal aortic aneurysm and their role in furthering management of human disease.</a> Cardiovasc Pathol. 20, 114-123 (2011).</li><li>Yamaguchi, T., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Factors+influencing+mortality+in+the+rat+elastase-induced-aneurysm+model.">Factors influencing mortality in the rat elastase-induced-aneurysm model.</a> J. Surg. Res. 94, 81-83 (2000).</li><li>von Bruhl, M. 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To modeller af AAA er allerede blevet beskrevet i vores lab: xenograft 19 og elastase inducerede modeller 1 Elastase model er den mest ligner menneskelige aortaaneurismer:. En stor omkredsen intraluminal blodprop derefter udelukkes ved endovaskulær aneurisme reparation. Endolækager er en sædvanlig komplikation af EVAR 14,15. Masser af spørgsmål forbliver uløste: AAA brud er blevet beskrevet med lave endolækager, og undertiden uden aneurismesækken udvi...

Endovaskulære færdigheder har gjort fremskridt siden den første endovaskulær aortaaneurisme reparation (EVAR) ved at Parodi i 1991 3, næsten halvdelen af alle abdominale aneurismer behandles nu af EVAR 4.. I modsætning kirurgi efterlader EVAR tromben, at udelukke det fra systemisk blodbanen. Aneurismer er kendetegnet ved en hæmoglobinkoncentration rige flere lag trombe, en tynd medier og en fibrosed og betændt adventitia. Den afgørende rolle for proteolyse 5 i aneurismet progression er påvist tredive år siden 6, 7: aneurismesækken trombe er forbundet med en tynd aortavæg, lysering af elastin, lav densitet af glatte muskelceller i medierne, og et højt niveau af immuno -inflammatorisk adventitiale reaktion 8,9. Disse ændringer tyder på, at proteolytisk enzymatisk aktivitet forekommer i tromben, snarere end direkte i aortavæggen. Desuden er det luminale lag af tromben rig på agglutinerede røde blodlegemer ceLLS frigiver hæmoglobin. De er primært involveret i dannelse af fibrin, blodpladeaktivering og inducere thrombin dannelse. Endelig trombe inducerer også t-PA og plasminogen fastholdelse, der er involveret i fibrinolyse og rekruttering leukocytter, primært neutrofile. Det er 12 gange mere vigtigt i blodprop end i blodet 10.. Deres tilstedeværelse er forbundet med en høj grad af matrixmetalloproteinase type 8 (MMP-8), MMP-9 og elastase: de frigiver granulære serinproteaser og pro oxidative midler fører til nedbrydning af fibrillære matrix, og i sidste ende aortavæggen briste 9,11 -13. Valgfag aneurisme indgreb har til formål at forhindre brud. EVAR efterlader både aneurisme væg og trombe intakt. Således er aneurisme perfusion af aorta sikkerheder (lumbal arterier, ringere mesenterialarterie ...), opkaldt endolækage, og er en specifik komplikation af endovaskulær behandling 14,15, undertiden fører til brud EveN med lavt tryk endolækager. I visse patienter, er der ingen stigning i aneurisme diameter 16. Interaktion mellem cirkulerende blod og aneurismal væg opretholder den nævnte intra luminale trombe biologisk aktivitet. Derfor, type V endolækager, defineret som udvidelse af aneurismesækken uden identificeret endolækage, kunne forklares ved enzymatisk aktivitet inde i aneurisme trombe. Hverken radiologisk billeddannelse (18FDG-PET scanning, blodpladeaktivering scintigrafi, jern oxyde kontrast MRI17) eller perifere blod prøvetagning (MMP-9, blodpladeafledte mikropartikler, plasmin / anti-plasmin kompleks), der bruges til at udforske den endovaskulære indvirkning på aortaaneurismer i mennesker direkte evaluere biokemiske veje. I vores viden, er der ingen nogen reproducerbare og billig eksperimentelle aneurisme udstødelse dyremodeller. Denne ene giver før og efter aneurisme udstødelse biologiske og histologiske ændringer, der skal udforskes.

<table border="1">
<tbody>
 <tr>
 <td></td>
 <td></td>
 <td></td>
 <td>Animals </td>
 </tr>
 <tr>
 <td>Wistar rats</td>
 <td></td>
 <td></td>
 <td>aged from 8 to 9 weeks. Weight range from 350 to 400 g</td>
 </tr>
 <tr>
 <td></td>
 <td></td>
 <td></td>
 <td>Instruments</td>
 </tr>
 <tr>
 <td>Isoflurane anesthesic system</td>
 <td></td>
 <td></td>
 <td>4,5% at the beginning, then 2%</td>
 </tr>
 <tr>
 <td>Penthobarbital</td>
 <td>Ceva sante animale, Libourne, France</td>
 <td></td>
 <td></td>
 </tr>
 <tr>
 <td>Dissection stereomicroscope</td>
 <td></td>
 <td></td>
 <td></td>
 </tr>
 <tr>
 <td>Steriles gloves</td>
 <td></td>
 <td></td>
 <td></td>
 </tr>
 <tr>
 <td>Microsurgical Steriles instruments</td>
 <td>Moria, Antony, France</td>
 <td></td>
 <td>Needle holder,	Forceps, Scissors,Gilbert approximator
o	3 Micro clamps</td>
 </tr>
 <tr>
 <td>N&deg;40 Silk string</td>
 <td></td>
 <td></td>
 <td></td>
 </tr>
 <tr>
 <td>Prolen 9-0 and 10-0</td>
 <td>Ethicon, Johnson &amp; Johnson, Auneau, France</td>
 <td></td>
 <td></td>
 </tr>
 <tr>
 <td>Heat-tapered polyethylene tubing</td>
 <td>PE 10</td>
 <td></td>
 <td></td>
 </tr>
 <tr>
 <td>Syringe infusion pump</td>
 <td></td>
 <td></td>
 <td></td>
 </tr>
 <tr>
 <td>Porcine pancreatic elastase</td>
 <td>Sigma, St. Louis, Mo., USA</td>
 <td></td>
 <td>550 &mu;l of salin is mixed with 175 &mu;l of elastase</td>
 </tr>
 <tr>
 <td>Indeflator</td>
 <td></td>
 <td></td>
 <td></td>
 </tr>
 <tr>
 <td>9 to 16 mm length, 3 mm diameter coronary PTFE covered stentgraft</td>
 <td>Abbott, Abbott Park, Illinois, USA</td>
 <td></td>
 <td></td>
 </tr>
 <tr>
 <td>C-Arm OEC 9800</td>
 <td>GE Medical Systems, Milwaukee, WI</td>
 <td></td>
 <td></td>
 </tr>
 <tr>
 <td>Aspegic 1000 mg</td>
 <td>Aspirin, Sanofi-Aventis, Paris, France</td>
 <td></td>
 <td></td>
 </tr>
 <tr>
 <td></td>
 <td></td>
 <td></td>
 <td>Reagent</td>
 </tr>
 <tr>
 <td>Aerane isoflurane 100ML</td>
 <td>Baxter</td>
 <td>Aerane</td>
 <td></td>
 </tr>
 <tr>
 <td>Penthobarbital</td>
 <td>Centravet</td>
 <td>053pen203</td>
 <td></td>
 </tr>
 <tr>
 <td>Silk Suture 4-0</td>
 <td>Fine Science</td>
 <td>18020-40</td>
 <td></td>
 </tr>
 <tr>
 <td>Microsurgical steriles instruments</td>
 <td>Moria</td>
 <td>9980-9983-9987-204/A-204/D-8148-4877A-4878A</td>
 <td></td>
 </tr>
 <tr>
 <td>Prolen 9-0 and 10-0</td>
 <td>Ethicon</td>
 <td>W2829 &ndash; NS2850</td>
 <td></td>
 </tr>
 <tr>
 <td>Heat tapered Polyethylene tubing : PE 10</td>
 <td>Bioseb</td>
 <td>MRE-010</td>
 <td></td>
 </tr>
 <tr>
 <td>Infusion pump</td>
 <td>World precision instrument</td>
 <td>AL-1000</td>
 <td></td>
 </tr>
 <tr>
 <td>Porcine pancreatic elastase</td>
 <td>Sigma</td>
 <td>E1250-100MG</td>
 <td></td>
 </tr>
 <tr>
 <td>Indeflator</td>
 <td>Longreal</td>
 <td>KY025 25 100</td>
 <td></td>
 </tr>
 <tr>
 <td>Aspegic 1000mg (Aspirin)</td>
 <td>Sanofi-Aventis</td>
 <td>3400931898191</td>
 <td></td>
 </tr>
 <tr>
 <td>C-Arm OEC 9800</td>
 <td>General electric.</td>
 <td>OEC 9800</td>
 <td></td>
 </tr>
 <tr>
 <td>Stentgraft : Jostent</td>
 <td>Abbott</td>
 <td>210CG1230 
 210CG1630</td>
 <td></td>
 </tr>
</tbody>
</table>

a new murine model of endovascular aortic aneurysm repair

Endovaskulær aneurisme udstødelse er en valideret teknik til at undgå aneurismebrud. Langsigtede resultater fremhæve teknik begrænsninger og nye aspekter af abdominalt aortaaneurisme (AAA) patofysiologi. Der er ingen abdominalt aortaaneurisme endograft udelukkelse model billig og reproducerbar, hvilket ville tillade dybe undersøgelser af AAA før og efter behandlingen. Vi hermed beskrive, hvordan at fremkalde og derefter at udelukke med en overdækket koronar stentgraft et abdominalt aortaaneurisme i en rotte. Den velkendte elastase induceret AAA model blev først rapporteret i 1990 1 i en rotte, så beskrevet i mus 2. Elastin nedbrydning fører til dilatation af aorta med inflammatorisk infiltration af bugvæggen og intra luminale trombe, der matcher med humant AAA. Endovaskulær udelukkelse med lille overdækket stentgraft udføres derefter, eksklusive eventuelle interaktioner mellem cirkulerende blod og aneurisme blodprop. Passende udstødelse og stentgraft patency erbekræftet før eutanasi af en angiografi troede venstre halspulsåre. Delvis kontrol elastase diffusion gør aneurisme form forskellig for hvert dyr. Det er vanskeligt at skabe en aneurisme, som vil give en passende længde af aorta under aneurismet for en nem stentgraft introduktion og med tilstrækkelig proksimal og distal hals for at forhindre endolækager. Masser af fejl kan føre til stentgraft introduktion, der til tider føre til aorta tåre med smerter og problemer at sy det, og endotel skade med posten op aorta trombose. Give aspirin til rotter før stentgraft implantation nedsætter fejlrate uden større blødning. Fastspænding tiden aktiverer neutrofile, endotel og blodplader, og kan interferere med biologisk analyse.

<html> Aneurisme er fastgjort i en paraformaldehydet 4% opløsning i løbet af 24 timer, og derefter ind i en phosphatbufret saltvandsopløsning indtil fiksering i en harpiks blok. Vi bruger en HPS (Hæmatoxylin Phloxin Safran) (Figur 1) farvning, viser healing proliferative celler omkring PTFE (Figur 2). Mesenchymceller derefter karakteriseret ved α-actin-farvning (figur 3). <img src="/files/ftp_upload/5...

Watch this Scientific Journal Video about A New Murine Model of Endovascular Aortic Aneurysm Repair at JoVE.com

A New Murine Model of Endovascular Aortic Aneurysm Repair

Histologiske og biokemiske ændringer efter aneurisme endograft udelukkelse er stadig uklart. Vi beskriver en ny model for endograft implantation på et murint aneurisme. Gennem blodprop analyser med og uden persistente cirkulerende blod stream interface, og nye endovaskulær biomaterialer evaluering, vil denne model bidrage til bedre at forstå endovaskulær aneurisme udstødelse Sygdomsbiologi.

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Open Tracheostomy Gastric Acid Aspiration Murine Model of Acute Lung Injury Results in Maximal Acute Nonlethal Lung Injury

Åbent Tracheostomituber Gastric Acid Aspiration murin model af akut lungeskade Resultater i Maximal Akut ikke-letale Lung Injury

Acid pneumonitis is a major cause of sterile acute lung injury (ALI) in humans. Acid pneumonitis spans the clinical spectrum from asymptomatic to acute ...

Reproducible Arterial Denudation Injury by Infrarenal Abdominal Aortic Clamping in a Murine Model

Reproducerbar arterielt blotlægning Skade af infrarenal Abdominal Aorta Opspænding i en murin model

Percutaneous vascular interventions uniformly result in arterial denudation injuries that subsequently lead to thrombosis and restenosis. These ...

Creation of a Rodent Model of Abdominal Aortic Aneurysm by Blocking Adventitial Vasa Vasorum Perfusion

Oprettelsen af en gnaver Model af abdominale aorta aneurisme ved at blokere Adventitial Vasa Vasorum Perfusion

The adventitial vasa vasorum (VV) provides oxygen and nourishment to the aortic wall. Hypoxia in the aortic wall can cause enlarged abdominal aortic ...

Quantitative Micro-CT Analysis of Aortopathy in a Mouse Model of &#946;-aminopropionitrile-induced Aortic Aneurysm and Dissection

Quantitative Micro-CT Analysis of Aortopathy in a Mouse Model of ÃƒÆ’Ã…Â½Ãƒâ€šÃ‚Â²-aminopropionitrile-induced Aortic Aneurysm and Dissection

Kvantitative mikro-CT-analyse af Aortopathy i en musemodel af β-aminopropionitrile-induceret aorta aneurisme og dissektion

Aortic aneurysm and dissection is associated with significant morbidity and mortality in the population and can be highly lethal. While animal models of ...

Murine Cervical Aortic Transplantation Model using a Modified Non-Suture Cuff Technique

Murine cervikale aorta transplantation model ved hjælp af en modificeret ikke-sutur manchet teknik

With the introduction of powerful immunosuppressive protocols, distinct advances are possible in the prevention and therapy of acute rejection episodes. ...

Murine Surgical Model of Topical Elastase Induced Descending Thoracic Aortic Aneurysm

Murine kirurgisk model af aktuel elastase induceret faldende Thoracic aorta aneurisme

According to the Center for Disease Control, aortic aneurysms (AAs) were considered a leading cause of death in all races and both sexes from 1999-2016. ...

Porcine Model of Infrarenal Abdominal Aortic Aneurysm

Svine model af Infrarenal abdominal aorta aneurisme

Large animal models to study abdominal aortic aneurysms are sparse. The purpose of this model is to create reproducible, clinically significant infrarenal ...