Sign In

A subscription to JoVE is required to view this content.

live

Speed

×

MEDIA_ELEMENT_ERROR: Format error

Yeast Luminometric and Xenopus Oocyte Electrophysiological Examinations of the Molecular Mechanosensitivity of TRPV4

10.0K Views

12:09 min

December 31st, 2013

December 31st, 2013

10,018 Views

0:05

Title

2:44

Yeast Luminometry Method

4:23

cRNA Injection

6:08

Two-electrode Voltage-clamp

8:11

Patch Clamp Examination of Direct Mechanosensitivity

9:41

Results: Yeast Luminometric and Xenopus Oocyte Electrophysiological Examinations of the Molecular Mechanosensitivity of TRPV4

11:38

Conclusion

To complement commonly used methods to study TRPV4’s, two methods are described: Its mechanosensitivity can be studied by Ca2+-aequorin luminometry in transgenic yeast upon hypo-osmotic challenge. It can also be examined in TRPV4-RNA injected Xenopus oocytes by whole-cell two-electrode voltage clamp or patch clamp in on-cell or excised mode.

We use cookies to enhance your experience on our website.

By continuing to use our website or clicking “Continue”, you are agreeing to accept our cookies.

Learn More