Ching Kung

Calmodulin as an ion channel subunit.

Gating the bacterial mechanosensitive channel MscL invivo.

An exchanger-like protein underlies the large Mg2+ current in Paramecium.

The transient receptor potential channel on the yeast vacuole is mechanosensitive.

PAK paradox: Paramecium appears to have more K(+)-channel genes than humans.

Gain-of-function mutations indicate that Escherichia coli Kch forms a functional K+ conduit in vivo.

Channels in microbes: so many holes to fill.

Heterologously expressed fungal transient receptor potential channels retain mechanosensitivity in vitro and osmotic response in vivo.

Microbial K+ channels.

Microbial mechanosensation.

Prokaryotic K(+) channels: from crystal structures to diversity.

A possible unifying principle for mechanosensation.

Lipid perturbations sensitize osmotic down-shock activated Ca2+ influx, a yeast "deletome" analysis.

Patch clamp and phenotypic analyses of a prokaryotic cyclic nucleotide-gated K+ channel using Escherichia coli as a host.

Yeast screens show aromatic residues at the end of the sixth helix anchor transient receptor potential channel gate.

Yeast gain-of-function mutations reveal structure-function relationships conserved among different subfamilies of transient receptor potential channels.

A genome-wide survey suggests an osmoprotective role for vacuolar Ca2+ release in cell wall-compromised yeast.

Indole and other aromatic compounds activate the yeast TRPY1 channel.

Ion channels in microbes.

Multiple unbiased prospective screens identify TRP channels and their conserved gating elements.

Hypotonic shocks activate rat TRPV4 in yeast in the absence of polyunsaturated fatty acids.

Mechanical force and cytoplasmic Ca(2+) activate yeast TRPY1 in parallel.

The use of yeast to understand TRP-channel mechanosensitivity.

Paramecium tetraurelia: the renaissance of an early unicellular model.

Maintaining clonal Paramecium tetraurelia cell lines of controlled age through daily reisolation.

Mass culture of Paramecium tetraurelia.

Silencing specific Paramecium tetraurelia genes by feeding double-stranded RNA.

DNA microinjection into the macronucleus of paramecium.

Immunocytochemistry of Paramecium cytoskeletal structures.

Forward genetic analysis reveals multiple gating mechanisms of TRPV4.

Wild-type and brachyolmia-causing mutant TRPV4 channels respond directly to stretch force.

Mechanosensitive channels in microbes.

Increased basal activity is a key determinant in the severity of human skeletal dysplasia caused by TRPV4 mutations.

The core domain as the force sensor of the yeast mechanosensitive TRP channel.

Stiffened lipid platforms at molecular force foci.